Construct: ORF TRCN0000474464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003140.1_s317c1
- Derived from:
- ccsbBroadEn_12143
- DNA Barcode:
- TGCACGGGCCGCTGCCTCTCCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTCD3 (55037)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55037 | PTCD3 | pentatricopeptide repeat do... | NM_017952.6 | 79.6% | 79.6% | 1_420del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1713
- ORF length:
- 1647
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgagtacttt gaacctcaga tcaaagacat aagtgaagcc gccctgaagg 121 aacgaattga gctcagaaaa gtcaaagcct ctgtggacat gtttgatcag cttttgcaag 181 caggaaccac tgtgtctctt gaaacaacaa atagtctctt ggatttattg tgttactatg 241 gtgaccagga gccctcaact gattaccatt ttcaacaaac tggacagtca gaagcattgg 301 aagaggaaaa tgatgagaca tctaggagga aagctggtca tcagtttgga gttacatggc 361 gagcaaaaaa caacgctgag agaatctttt ctctaatgcc agagaaaaat gaacattcct 421 attgcacaat gatccgagga atggtgaagc accgagctta tgagcaggca ttaaacttgt 481 acactgagtt actaaacaac agactccatg ctgatgtata cacatttaat gcattgattg 541 aagcaacagt atgtgcgata aatgagaaat ttgaggaaaa atggagtaaa atactggagc 601 tgctaagaca catggttgca cagaaggtga aaccaaatct tcagactttt aataccattc 661 tgaaatgtct ccgaagattt catgtgtttg caagatcgcc agccttacag gttttacgtg 721 aaatgaaagc cattggaata gaaccctcgc ttgcaacata tcaccatatt attcgcctgt 781 ttgatcaacc tggagaccct ttaaagagat catccttcat catttatgat ataatgaatg 841 aattaatggg aaagagattt tctccaaagg acccggatga tgataagttt tttcagtcag 901 ccatgagcat atgctcatct ctcagagatc tagaacttgc ctaccaagta catggccttt 961 taaaaaccgg agacaactgg aaattcattg gacctgatca acatcgtaat ttctattatt 1021 ccaagttctt cgatttgatt tgtctaatgg aacaaattga tgttaccttg aagtggtatg 1081 aggacctgat accttcagcc tactttcccc actcccaaac aatgatacat cttctccaag 1141 cattggatgt ggccaatcgg ctagaagtga ttcctaaaat ttggaaagat agtaaagaat 1201 atggtcatac tttccgcagt gacctgagag aagagatccT GATGCTCATG GCAAGGGACA 1261 AGCACCCACC AGAGCTTCAG GTGGCATTTG CTGACTGTGC TGCTGATATC AAATCTGCGT 1321 ATGAAAGCCA ACCCATCAGA CAGACTGCTC AGGATTGGCC AGCCACCTCT CTCAACTGTA 1381 TAGCTATCCT CTTTTTAAGG GCTGGGAGAA CTCAGGAAGC CTGGAAAATG TTGGGGCTTT 1441 TCAGGAAGCA TAATAAGATT CCTAGAAGTG AGTTGCTGAA TGAGCTTATG GACAGTGCAA 1501 AAGTGTCTAA CAGCCCTTCC CAGGCCATTG AAGTAGTAGA GCTGGCAAGT GCCTTCAGCT 1561 TACCTATTTG TGAGGGCCTC ACCCAGAGAG TAATGAGTGA TTTTGCAATC AACCAGGAAC 1621 AAAAGGAAGC CCTAAGTAAT CTAACTGCAT TGACCAGTGA CAGTGATACT GACAGCAGCA 1681 GTGACAGCGA CAGTGACACC AGTGAAGGCA AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 1741 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1801 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1861 CACGGGCCGC TGCCTCTCCG CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1921 ttgtgaaaga tt