Construct: ORF TRCN0000474637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010864.1_s317c1
- Derived from:
- ccsbBroadEn_15855
- DNA Barcode:
- AACTATCCATCCACCTATGAGCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SELENOT (51714)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474637
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51714 | SELENOT | selenoprotein T | NM_016275.5 | 83.5% | 33.3% | 1_96del |
| 2 | mouse | 69227 | Selenot | selenoprotein T | NM_001040396.3 | 80.1% | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 114
- ORF length:
- 48
- Sequence:
-
1 tcttccattt cagGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCA GTACGCCACG GGGCCGCTGC TCAAGTTCCA GATTTGTGTT TCCTGAGGTT 121 ATAGGCGGGT GTTTGAGGAG TACATGCGGG TTATTAGCCA GCGGTACCCA GACATCCGCA 181 TTGAAGGAGA GAATTACCTC CCTCAACCAA TATATAGACA CATAGCATCT TTCCTGTCAG 241 TCTTCAAACT AGTATTAATA GGCTTAATAA TTGTTGGCAA GGATCCTTTT GCTTTCTTTG 301 GCATGCAAGC TCCTAGCATC TGGCAGTGGG GCCAAGAAAA TAAGGTTTAT GCATGTATGA 361 TGGTTTTCTT CTTGAGCAAC ATGATTGAGA ACCAGTGTAT GTCAACAGGT GCATTTGAGA 421 TAACTTTAAA TGATGTACCT GTGTGGTCTA AGCTGGAATC TGGTCACCTT CCATCCATGC 481 AACAACTTGT TCAAATTCTT GACAATGAAA TGAAGCTCAA TGTGCATATG GATTCAATCC 541 CACACCATCG ATCAAACCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 601 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 661 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ACTATCCATC CACCTATGAG 721 CGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att