Construct: ORF TRCN0000474654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003736.1_s317c1
- Derived from:
- ccsbBroadEn_07302
- DNA Barcode:
- CCCCCAGAAAGATGCCTATCTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FPGT (8790)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8790 | FPGT | fucose-1-phosphate guanylyl... | NM_003838.4 | 97.8% | 97.8% | 1_39del;267G>A |
| 2 | human | 8790 | FPGT | fucose-1-phosphate guanylyl... | NM_001199328.2 | 55.9% | 56% | 1_39del;267G>A;447_448ins762 |
| 3 | human | 8790 | FPGT | fucose-1-phosphate guanylyl... | NM_001199329.2 | 27.7% | 19.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1848
- ORF length:
- 1782
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agctgctagg gaccctccgg aagtatcgct gcgagaagcc acccagcgaa 121 aattgcggag gttttccgag ctaagaggca aacttgtagc acgtggagaa ttctgggaca 181 tagttgcaat aacagcggct gatgaaaaac aggaacttgc ttacaaccaa cagctgtcag 241 aaaagctgaa aagaaaggag ttaccccttg gagttcaata tcacgttttt gtagatcctg 301 ctggagccaa aattggaaat ggaggatcaa cactttgtgc ccttcaatgt ttggaaaagc 361 tatatggaga taaatggaat tcttttacca tcttattaat tcactctggt ggctacagtc 421 aacgacttcc aaatgcaagt gctctgggaa aaattttcac tgctttacct cttggtaacc 481 ccatttatca gatgctagaa ttaaaactag ccatgtacat tgatttcccc ttaaatatga 541 atcctggaat tctggttacc tgtgcagatg atattgaact ttatagtatt ggagaatttg 601 agtttattag gtttgacaaa cctggcttta ctgctttagc tcatccttct agtttgacga 661 taggtaccac acatggagta tttgtcttag atccttttga tgatttaaaa catagagacc 721 ttgaatacag gtcttgccat cgtttccttc ataagcccag catagaaaag atgtatcagt 781 ttaatgctgt gtgtagacct ggaaattttt gtcaacagga ctttgctggg ggtgacattg 841 ccgatcttaa attagactct gactatgtct acacagatag cctattttat atggatcata 901 aatcagcaaa aatgttactt gctttttatg aaaaaatagg cacactgagc tgtgaaatag 961 atgcctatgg tgactttctg caggctttgg gacctggagc aactgtggag tacaccagaa 1021 acacatcaaa tgtcattaaa gaagagtcag agttggtaga aatgaggcag agaatatttc 1081 atcttcttaa aggaacatca ctaaatgttg ttgttcttaa taactccaaa ttttatcaca 1141 ttggaacaac cgaagaatat ttgttttact ttacctcaga taacagttta aagtcagagc 1201 tcggcttaca gtccataact tttagtatct ttccagatat accagaatgc tctggcaaaa 1261 catcctgtat cattcaaagc atactggatt caagatgttc tgtggcacct ggctcagttg 1321 tggagtattc cagattgggg cctgatgttt cagttgggga aaactgcatt attagtggtt 1381 cttacatcct aacaaaagct gccctccccg cacattcttt tgtatgttcc TTAAGCTTAA 1441 AGATGAATAG ATGCTTAAAG TATGCAACTA TGGCATTTGG AGTGCAAGAC AACTTGAAAA 1501 AGAGTGTGAA AACATTGTCA GATATAAAGT TACTTCAATT CTTTGGAGTC TGTTTCCTGT 1561 CATGCTTAGA TGTTTGGAAT CTTAAAGTTA CAGAGGAACT GTTCTCTGGT AACAAGACAT 1621 GTCTGAGTTT GTGGACTGCA CGCATTTTCC CAGTTTGTTC TTCTTTGAGT GACTCAGTTA 1681 TAACATCCCT AAAGATGTTA AATGCTGTTA AGAACAAGTC AGCATTCAGC CTGAATAGCT 1741 ATAAGTTGCT GTCCATTGAA GAAATGCTTA TCTACAAAGA TGTAGAAGAT ATGATAACTT 1801 ACAGGGAACA AATTTTTCTA GAAATCAGTT TAAAAAGCAG TTTGATGTAC CCGACTTTCT 1861 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1921 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1981 GTGGAAAGGA CGACCCCCAG AAAGATGCCT ATCTCAAACG CGTTAAGTCg acaatcaacc 2041 tctggattac aaaatttgtg aaagatt