Construct: ORF TRCN0000474655

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004770.1_s317c1
Derived from:
ccsbBroadEn_15221
DNA Barcode:
CGCATTACAACGTATGGTGATACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADAT3 (113179)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474655
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 113179 ADAT3 adenosine deaminase tRNA sp... NM_001329533.2 100% 100%
2 human 113179 ADAT3 adenosine deaminase tRNA sp... NM_138422.4 95.6% 95.6% 1_48del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1119
ORF length:
1053
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gcccgccccg ggcctcgtgg agcagcccaa gtgcttggag gccgggagcc
121cggagcctga gccggcgccg tggcaggccc tccctgtcct gtccgagaag cagtcagggg
181acgtggagct ggtgctggcc tacgccgcgc ccgtcctgga caagcgccag acctcacgcc
241tcctgaagga ggtgtcggcc ctgcacccgc tccccgccca gcctcacctc aagcgggtgc
301ggcccagccg cgatgccggc agcccccacg ccctggagat gctgctttgc ctggctgggc
361cggcctcggg cccgcgctcg ctggctgagc tcctgccacg gccggctgtg gacccccgcg
421gcctggggca acccttcctg gtgcccgtgc ccgcccggcc gcctctgacc aggggccagt
481tcgaggaggc ccgggcccac tggcccacgt ccttccacga ggacaagcag gtgaccagcg
541ccctggctgg gcggctcttc tccacgcagg agcgcgccgc catgcagagc cacatggagc
601gggcggtgtg ggcggcccgg cgggcagcag cgcggggctt gcgggccgtg ggggccgtgg
661tagtggaccc ggcctcggac cgcgtgctgg ccaccggcca cgactgcagc tgcgcggaca
721accccctcct gcacgccgtc atggtgtgcg tggacctcgt ggcgcgcggc cagggccgcg
781gcacctacga cttcagaccc ttccccgcct gctccTTCGC CCCGGCCGCT GCCCCCCAGG
841CCGTCCGCGC AGGCGCCGTG CGTAAACTGG ACGCAGACGA GGACGGCCTC CCCTACCTGT
901GCACTGGCTA CGACCTGTAC GTGACCCGCG AGCCCTGCGC CATGTGCGCC ATGGCCCTGG
961TGCACGCACG CATCCTGCGC GTCTTCTACG GTGCGCCCTC GCCCGACGGC GCCCTGGGCA
1021CCCGCTTCCG CATCCACGCA CGGCCCGACC TCAACCACCG CTTCCAGGTG TTCCGCGGGG
1081TGCTGGAGGA GCAGTGCCGC TGGCTGGACC CCGACACGTA CCCAACTTTC TTGTACAAAG
1141TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1201GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1261ACGACGCATT ACAACGTATG GTGATACCAC GCGTTAAGTC gacaatcaac ctctggatta
1321caaaatttgt gaaagatt

Download FASTA (ORF) (Full)