Construct: ORF TRCN0000474669
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001753.1_s317c1
- Derived from:
- ccsbBroadEn_00666
- DNA Barcode:
- GACGAGAGTAAGTACGCTCCCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPD1 (2819)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474669
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2819 | GPD1 | glycerol-3-phosphate dehydr... | NM_005276.4 | 100% | 100% | |
| 2 | human | 2819 | GPD1 | glycerol-3-phosphate dehydr... | NM_001257199.2 | 93.4% | 93.4% | 218_219ins69 |
| 3 | mouse | 14555 | Gpd1 | glycerol-3-phosphate dehydr... | NM_010271.2 | 89.6% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tagcaagaaa gtctgcattg taggctccgg gaactggggc tcagccatcg 121 ccaagatcgt gggtggcaat gcagcccagc tggcacagtt tgacccacgg gtgaccatgt 181 gggtatttga ggaagacatt ggaggcaaaa agctgactga gatcatcaac acgcagcatg 241 agaatgtcaa atacctgcca gggcacaagt tgcccccaaa tgtggtggct gtcccagatg 301 tggtccaggc tgcagaggat gctgacatcc tgatctttgt ggtgccccat cagttcatcg 361 gcaagatctg tgaccagctc aagggccatc tgaaggcaaa cgccactggc atatctctta 421 ttaagggggt agacgagggc cccaatgggc tgaagctcat ctcggaagtg attggggagc 481 gcctcggcat ccccatgagt gtgctgatgg gggccaacat tgccagcgag gtggctgatg 541 agaagttctg tgagacaacc attggctgca aggacccggc ccagGGACAA CTCCTGAAAG 601 AGCTGATGCA GACACCAAAC TTCCGTATCA CAGTGGTGCA AGAGGTGGAC ACAGTAGAGA 661 TCTGTGGAGC CTTAAAGAAT GTAGTGGCCG TGGGGGCTGG CTTCTGTGAT GGCCTGGGCT 721 TTGGCGACAA CACCAAGGCG GCAGTGATCC GGCTGGGACT CATGGAGATG ATAGCCTTCG 781 CCAAGCTCTT CTGCAGTGGC CCTGTGTCCT CTGCCACCTT CTTGGAGAGC TGTGGTGTTG 841 CTGACCTGAT CACTACCTGC TATGGAGGGC GGAACCGGAA AGTGGCTGAG GCCTTTGCGC 901 GTACAGGAAA GTCCATTGAG CAGCTGGAGA AAGAGTTGCT GAATGGGCAG AAACTGCAGG 961 GGCCCGAGAC AGCCCGGGAG CTATACAGCA TCCTCCAGCA CAAGGGCCTG GTAGACAAGT 1021 TTCCCTTGTT CATGGCTGTG TACAAGGTGT GCTACGAGGG CCAGCCAGTG GGTGAATTCA 1081 TCCACTGCCT GCAGAATCAT CCAGAACATA TGTGCCCAAC TTACTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 1261 CGAGAGTAAG TACGCTCCCG TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt