Construct: ORF TRCN0000474685
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004003.1_s317c1
- Derived from:
- ccsbBroadEn_07121
- DNA Barcode:
- ATTGATAACGTATCATTAGACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR1H2 (7376)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474685
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7376 | NR1H2 | nuclear receptor subfamily ... | NM_007121.7 | 99.7% | 99.5% | 7T>G;516_517insCAG |
| 2 | human | 7376 | NR1H2 | nuclear receptor subfamily ... | NM_001256647.2 | 78.6% | 78.3% | 7T>G;181_182ins291;225_226insCAG |
| 3 | mouse | 22260 | Nr1h2 | nuclear receptor subfamily ... | NM_001285517.1 | 83.2% | 87.2% | (many diffs) |
| 4 | mouse | 22260 | Nr1h2 | nuclear receptor subfamily ... | NM_009473.3 | 83.2% | 87.2% | (many diffs) |
| 5 | mouse | 22260 | Nr1h2 | nuclear receptor subfamily ... | XM_006540804.3 | 83.2% | 87.2% | (many diffs) |
| 6 | mouse | 22260 | Nr1h2 | nuclear receptor subfamily ... | NM_001285518.1 | 82.7% | 87% | (many diffs) |
| 7 | mouse | 22260 | Nr1h2 | nuclear receptor subfamily ... | NM_001285519.1 | 82.7% | 87% | (many diffs) |
| 8 | mouse | 22260 | Nr1h2 | nuclear receptor subfamily ... | XM_017322126.1 | 71.6% | 76.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1449
- ORF length:
- 1383
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgctcctacc acgagttccc tggatacccc cctgcctgga aatggccccc 121 ctcagcctgg cgccccttct tcttcaccca ctgtaaagga ggagggtccg gagccgtggc 181 ccgggggtcc ggaccctgat gtcccaggca ctgatgaggc cagctcagcc tgcagcacag 241 actgggtcat cccagatccc gaagaggaac cagagcgcaa gcgaaagaag ggcccagccc 301 cgaagatgct gggccacgag ctttgccgtg tctgtgggga caaggcctcc ggcttccact 361 acaacgtgct cagctgcgaa ggctgcaagg gcttcttccg gcgcagtgtg gtccgtggtg 421 gggccaggcg ctatgcctgc cggggtggcg gaacctgcca gatggacgct ttcatgcggc 481 gcaagtgcca gcagtgccgg ctgcgcaagt gcaaggaggc agggatgagg gagcagtgcg 541 tcctttctga agaacagatc cggaagaaga agattcggaa acagcagcag caggagtcac 601 agtcacagtc gcagtcacct gtggggccgc agggcagcag cagctcagcc tctgggcctg 661 gggcttcccc tggtggatct gaggcaggca gccagggctc cggggaaggc gagggtgtcc 721 agctaacagc ggctcaagaa ctaatgatcc agcagttggt ggcggcccaa ctgcagtgca 781 acaaacgctc cttctccgac cagcccaaag tcacgccctg gcccctgggc gcagaccccc 841 agtcccgaga tgcccgccag caacgctttg cccacttcac ggagctggcc atcatctcag 901 tccaggagat cgtggacttc gctaagcaag tgcctggttt cctgcagctg ggccgggagg 961 accagatcgc cctcctgaag gcatccacta tcgagatcat gctgctagag acagccaggc 1021 gctacaacca cgagacagag tgtatcacct tcttgaagga cttcaccTAC AGCAAGGACG 1081 ACTTCCACCG TGCAGGCCTG CAGGTGGAGT TCATCAACCC CATCTTCGAG TTCTCGCGGG 1141 CCATGCGGCG GCTGGGCCTG GACGACGCTG AGTACGCCCT GCTCATCGCC ATCAACATCT 1201 TCTCGGCCGA CCGGCCCAAC GTGCAGGAGC CGGGCCGCGT GGAGGCGTTG CAGCAGCCCT 1261 ACGTGGAGGC GCTGCTGTCC TACACGCGCA TCAAGAGGCC GCAGGACCAG CTGCGCTTCC 1321 CGCGCATGCT CATGAAGCTG GTGAGCCTGC GCACGCTGAG CTCTGTGCAC TCGGAGCAGG 1381 TCTTCGCCTT GCGGCTCCAG GACAAGAAGC TGCCGCCTCT GCTGTCGGAG ATCTGGGACG 1441 TCCACGAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1501 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1561 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATTGAT AACGTATCAT TAGACCGCAC 1621 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt