Construct: ORF TRCN0000474749
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006548.1_s317c1
- Derived from:
- ccsbBroadEn_04284
- DNA Barcode:
- CAATCATTAAATAGCCCAACCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAMAC (83640)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474749
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83640 | RAMAC | RNA guanine-7 methyltransfe... | NM_031452.4 | 100% | 100% | |
| 2 | mouse | 67148 | Fam103a1 | family with sequence simila... | NM_025997.2 | 84.6% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 444
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGACTGACAC TGCCGAAGCT GTTCCAAAGT 121 TTGAAGAGAT GTTTGCTAGT AGATTCACAG AAAATGACAA GGAGTATCAG GAATACCTGA 181 AACGCCCTCC TGAGTCTCCT CCAATTGTTG AGGAATGGAA TAGCAGAGCT GGTGGGAACC 241 AAAGAAACAG AGGCAATCGG TTGCAAGACA ACAGACAGTT CAGAGGCAGG GACAACAGAT 301 GGGGGTGGCC AAGTGACAAT CGATCCAATC AGTGGCATGG ACGATCCTGG GGTAACAACT 361 ACCCGCAACA CAGACAAGAA CCTTACTATC CCCAGCAATA TGGACATTAT GGTTACAACC 421 AGCGGCCTCC TTACGGTTAC TACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AATCATTAAA 601 TAGCCCAACC TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att