Construct: ORF TRCN0000474793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012474.1_s317c1
- Derived from:
- ccsbBroadEn_00339
- DNA Barcode:
- CCACTCGTACATGGAGGCACACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNN3 (1266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1266 | CNN3 | calponin 3 | NM_001839.5 | 100% | 100% | |
| 2 | human | 1266 | CNN3 | calponin 3 | NM_001286056.1 | 87.5% | 87.5% | 0_1ins123 |
| 3 | human | 1266 | CNN3 | calponin 3 | XM_017000245.2 | 87.5% | 87.5% | 0_1ins123 |
| 4 | human | 1266 | CNN3 | calponin 3 | NM_001286055.1 | 86% | 86% | 245_246ins138 |
| 5 | mouse | 71994 | Cnn3 | calponin 3, acidic | NM_028044.2 | 89.8% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1053
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ccacttcaac aagggccctt cctatgggct ctcggccgaa gtcaagaaca 121 agattgcttc caagtatgat catcaggcag aagaagatct tcgcaattgg atagaagagg 181 tgacaggcat gagcattggc cccaacttcc agctgggctt aaaggatggc atcatcctct 241 gcgaacttat aaacaagcta cagccaggct cagtgaagaa ggtcaacgag tcctcactga 301 actggcctca gttggagaat attggcaact ttattaaagc tattcaggct tatggtatga 361 agccacatga catattcgaa gcaaatgatc tttttgagaa tggaaacatg acccaggttc 421 agactactct ggtggctcta gcaggtctgg ctaaaacaaa aggattccat acaaccattg 481 acattggagt taagtatGCA GAAAAACAAA CAAGACGTTT TGATGAAGGA AAATTAAAAG 541 CTGGCCAAAG TGTAATTGGT CTGCAGATGG GAACCAACAA ATGTGCCAGC CAGGCAGGTA 601 TGACAGCTTA CGGGACTAGG AGGCATCTTT ATGATCCCAA AATGCAAACT GACAAACCTT 661 TTGACCAGAC CACAATTAGT CTGCAGATGG GCACTAATAA AGGAGCCAGC CAGGCAGGGA 721 TGTTAGCACC AGGTACCAGA AGAGACATCT ATGATCAGAA GCTAACATTA CAGCCGGTGG 781 ACAACTCGAC AATTTCCCTA CAGATGGGTA CCAACAAAGT TGCTTCCCAG AAAGGAATGA 841 GTGTGTATGG GCTTGGGCGG CAAGTATATG ATCCCAAATA CTGTGCTGCT CCTACAGAAC 901 CTGTCATTCA CAACGGAAGC CAAGGAACAG GAACAAATGG TTCGGAAATC AGTGATAGTG 961 ATTATCAGGC AGAATACCCT GATGAGTATC ATGGCGAGTA CCAGGATGAC TACCCCAGAG 1021 ATTACCAATA TAGCGACCAA GGCATTGATT ATTACCCAAC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1201 ACTCGTACAT GGAGGCACAC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt