Construct: ORF TRCN0000474808
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006715.1_s317c1
- Derived from:
- ccsbBroadEn_10369
- DNA Barcode:
- AGCGGGGTTGACCGATCATTTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGES2-AS1 (389791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474808
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389791 | PTGES2-AS1 | PTGES2 antisense RNA 1 (hea... | NR_024425.1 | 26.1% | 1_116del;672_2121del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 624
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GCCGGTGAAG ACGAGAGCGG AAGGCGAGGA CGACGGCTTC GGGGAAGCGG 121 GTGACCCGAG GAGACTGCTG GAGCGACCTT GGCGTTTCCG GGGGTGCCTT CCCGGGAAGG 181 GGAATCGGGA TGTTGGCTTC GAAGGGACCG AAGGGCCCAC CTCGACCCGC CCCGAGTGGG 241 TTTGGAGTTG TAGGTGCTGT CTCGGATGCA GGGCGAGCAC CCGCGAGCGG GTAACCAGTC 301 CCGTGAGAGC TGCAGGTCCC CAGCCCCGTT TTACAGATAG GGAAACTGAG GCAGCTGCTG 361 GGACACTCGC CCATATGGGT TTTGCTCCAC CCACTTCCTT CAGCCACTTT ACAGACCAGG 421 AGTTGAGGGA CTGCTCGAGC TTAGAGTGTT TAGGGGTGGT TGAGGGTGAC CCCCATGTTC 481 TGTGCTCTAC TCTATCCCTA TCCAGGCCTT CTCCCAGCGC CACTCTCACG CTCCTGCTTG 541 CTAGCTCCTG CTTGCTAGCT CCTGCTCCTC CCAGCTTCAT CCTCCTCCTG TTTACATTAA 601 TTGCTCCTGA TCTTCCACAC TCTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 661 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 721 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCGGGGTTGA 781 CCGATCATTT CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 841 att