Construct: ORF TRCN0000474819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006068.1_s317c1
- Derived from:
- ccsbBroadEn_05262
- DNA Barcode:
- GGCCGATAACGGACTCATGCCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AC1 (221613)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221613 | H2AC1 | H2A clustered histone 1 | NM_170745.3 | 100% | 100% | |
| 2 | human | 8334 | H2AC6 | H2A clustered histone 6 | NM_003512.3 | 80.4% | 93.8% | (many diffs) |
| 3 | human | 8329 | H2AC13 | H2A clustered histone 13 | NM_003509.2 | 79.4% | 93.1% | (many diffs) |
| 4 | human | 8331 | H2AC14 | H2A clustered histone 14 | NM_021066.2 | 77.7% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 89
- ORF end:
- 482
- ORF length:
- 393
- Sequence:
-
1 CAGACAGTGG TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG 61 ATCAACAAGT TTGTACAAAA AAGTTGGCAT GTCTGGACGA GGGAAGCAGG GAGGAAAAGC 121 ACGCGCCAAG TCTAAGTCTC GCTCTTCTAG AGCGGGTTTG CAGTTTCCCG TAGGCCGGAT 181 CCATCGTCTG CTTCGTAAGG GAAACTATGC AGAGCGGATA GGGGCAGGCG CACCAGTGTA 241 TTTGGCGGCA GTGTTAGAGT ATCTCACAGC AGAAATCCTT GAGCTGGCAG GCAATGCGTC 301 TCGCGATAAC AAAAAAACTC GCATTATTCC CCGCCACCTG CAGCTAGCGA TCCGCAATGA 361 TGAGGAACTC AATAAGCTTT TGGGCGGCGT GACCATTGCC CAGGGCGGAG TCCTGCCTAA 421 CATTCAGGCA GTGCTGCTGC CCAAGAAGAC TGAGAGTCAC CACCATAAAG CCCAAAGCAA 481 GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 541 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 601 GCTTTATATA TCTTGTGGAA AGGACGAGGC CGATAACGGA CTCATGCCCT AACGCGTTAA 661 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t