Construct: ORF TRCN0000474839
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018269.1_s317c1
- Derived from:
- ccsbBroadEn_02411
- DNA Barcode:
- ACCCGTAGAAGCGAGCAATCCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNG3 (10368)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474839
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10368 | CACNG3 | calcium voltage-gated chann... | NM_006539.4 | 100% | 100% | |
| 2 | mouse | 54376 | Cacng3 | calcium channel, voltage-de... | NM_019430.2 | 90.5% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gatgtgtgac agaggtatcc agatgttgat caccactgta ggagcctttg 121 ccgcttttag tttaatgacc attgcagtgg gcacggacta ctggttatat tccagaggtg 181 tgtgcaggac taaatctaca agtgataatg aaaccagcag gaagaatgaa gaagtaatga 241 cccattcggg gctgtggagg acctgctgcc tagaaggggc tttccgaggc gtgtgcaaga 301 aaatcgatca cttccctgaa gatgctgact acgaacagga cacagccgaa tatctcctgc 361 gagctgtgag ggcctccagt gtcttcccca tcctcagtgt cacgctgctg ttcttcggcg 421 ggctctgcgt ggcagccagt gagttccacc gcagcagaca caacgtcatt cTCAGCGCGG 481 GCATCTTTTT TGTCTCTGCA GGGTTAAGCA ACATCATTGG CATCATAGTT TATATATCAG 541 CCAACGCCGG AGACCCCGGG CAGCGTGACT CCAAAAAAAG TTACTCCTAT GGTTGGTCCT 601 TTTATTTCGG AGCCTTCTCT TTCATCATCG CAGAAATTGT AGGAGTGGTT GCCGTGCACA 661 TCTATATTGA AAAACATCAG CAGTTACGAG CCAAATCCCA CTCGGAGTTC CTGAAGAAAT 721 CTACTTTTGC CCGCCTCCCA CCCTACAGGT ATCGATTCCG GAGGCGGTCA AGTTCTCGCT 781 CCACCGAGCC CAGATCCCGA GACCTGTCCC CCATCAGCAA AGGCTTCCAC ACCATCCCTT 841 CCACTGACAT CTCGATGTTC ACCCTCTCCC GGGACCCCTC AAAGATCACC ATGGGGACCC 901 TCCTCAACTC CGACCGGGAC CACGCTTTTC TACAGTTCCA CAATTCCACA CCCAAAGAGT 961 TCAAAGAGTC ACTGCATAAT AATCCGGCCA ACAGGCGCAC CACGCCCGTC TGCCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAACCC GTAGAAGCGA GCAATCCCTT ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt