Construct: ORF TRCN0000474894
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018112.1_s317c1
- Derived from:
- ccsbBroadEn_10382
- DNA Barcode:
- CTATCGTAACAAGTGACTCTGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXB-AS3 (404266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474894
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 404266 | HOXB-AS3 | HOXB cluster antisense RNA 3 | NR_033204.2 | 30.9% | 1_154del;314_514del | |
| 2 | human | 404266 | HOXB-AS3 | HOXB cluster antisense RNA 3 | NR_033201.2 | 26% | 1_251del;411_611del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 285
- ORF end:
- 444
- ORF length:
- 159
- Sequence:
-
1 GGCGCCGTCC AGGCACCTCG ATTAGTTCTC GAGCTTTTGG AGTACGTCGT CTTTAGGTTG 61 GGGGGAGGGG TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG 121 GCCAGCTTGG CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG 181 GTTCATTCTC AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG 241 AGGCTAGCAT CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCATGCCA GTGCTGCCGG 301 GAACCCAGCG ATATCCGCAC CAGCGGAGAA GGTTCCAGGC TGCCGGCGGC GGCGCAGAGA 361 GCGGGAAGAG AGGCTCGGAG GAAGCCCCGG GCGTGGCGTG GTCAGGCTCC GAGAGCGGCC 421 GGGATGCGGC CACACCGGCC TGGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TATCGTAACA 601 AGTGACTCTG AGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att