Construct: ORF TRCN0000474943

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007308.1_s317c1
Derived from:
ccsbBroadEn_00164
DNA Barcode:
ACAGTTGATATCCTCAAGATTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BLMH (642)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474943
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 642 BLMH bleomycin hydrolase NM_000386.4 100% 100%
2 mouse 104184 Blmh bleomycin hydrolase NM_178645.4 91.1% 93.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1431
ORF length:
1365
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag cagctcggga ctgaattcgg agaaggtagc tgctctgata cagaaactga
121attccgaccc ccagttcgta cttgcccaga atgtcgggac cacccacgac ctgctggaca
181tctgtctgaa gcgggccacg gtgcagcgcg cgcagcatgt gttccagcac gccgtgcccc
241aggagggcaa gccaatcacc aaccagaaga gctcagggcg atgctggatc ttttcttgtc
301tgaatgttat gaggcttcca ttcatgaaaa agttaaatat tgaagaattt gagtttagcc
361aatcttacct gtttttttgg gacaaggttg aacgctgtta tttcttcttg agtgcttttg
421tggacacagc ccagagaaag gagcctgagg atgggaggct ggtgcagttt ttgcttatga
481accctgcaaa tgatggtggc caatgggata tgcttgttaa tattgttgaa aaatatggtg
541ttatccctaa gaaatgcttc cctgaatctt atacaacaga ggcaaccaga aggatgaatg
601atattctgaa tcacaagatg agagaattct gtatacgact gcggaacctg gtacacagtg
661gagcaaccaa aggagaaatc tcggccacac aggacgtcat gatggaggag atattccgag
721tggtgtgcat ctgtttgggt aatccaccag agacattcac ctgggaatat cgagacaaag
781ataaaaatta tcagaaaatt ggccccataa cacccttgga gttttacagg gaacatgtCA
841AGCCACTCTT CAATATGGAA GATAAGATTT GTTTAGTGAA TGACCCTAGG CCCCAGCACA
901AGTACAACAA ACTTTACACA GTGGAATACT TAAGCAATAT GGTTGGAGGG AGAAAAACTC
961TATACAACAA CCAGCCCATT GACTTCCTGA AAAAGATGGT TGCTGCCTCC ATCAAAGATG
1021GAGAGGCTGT GTGGTTTGGC TGTGATGTTG GAAAACACTT CAATAGCAAG CTGGGCCTCA
1081GTGACATGAA TCTCTATGAC CATGAGTTAG TGTTTGGTGT CTCCTTGAAG AACATGAATA
1141AAGCGGAGAG GCTGACTTTT GGTGAGTCAC TTATGACCCA CGCCATGACC TTCACTGCTG
1201TCTCAGAGAA GGATGATCAG GATGGTGCTT TCACAAAATG GAGAGTGGAG AATTCATGGG
1261GTGAAGACCA TGGCCACAAA GGTTACCTGT GCATGACAGA TGAGTGGTTC TCTGAGTATG
1321TCTACGAAGT GGTGGTGGAC AGGAAGCATG TCCCTGAAGA GGTGCTAGCT GTGTTAGAGC
1381AGGAACCCAT TATCCTGCCA GCATGGGACC CCATGGGAGC TTTGGCTGAG TGCCCAACTT
1441TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1501CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1561CTTGTGGAAA GGACGAACAG TTGATATCCT CAAGATTTTC ACGCGTTAAG TCgacaatca
1621acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)