Construct: ORF TRCN0000474943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007308.1_s317c1
- Derived from:
- ccsbBroadEn_00164
- DNA Barcode:
- ACAGTTGATATCCTCAAGATTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BLMH (642)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474943
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 642 | BLMH | bleomycin hydrolase | NM_000386.4 | 100% | 100% | |
| 2 | mouse | 104184 | Blmh | bleomycin hydrolase | NM_178645.4 | 91.1% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1431
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cagctcggga ctgaattcgg agaaggtagc tgctctgata cagaaactga 121 attccgaccc ccagttcgta cttgcccaga atgtcgggac cacccacgac ctgctggaca 181 tctgtctgaa gcgggccacg gtgcagcgcg cgcagcatgt gttccagcac gccgtgcccc 241 aggagggcaa gccaatcacc aaccagaaga gctcagggcg atgctggatc ttttcttgtc 301 tgaatgttat gaggcttcca ttcatgaaaa agttaaatat tgaagaattt gagtttagcc 361 aatcttacct gtttttttgg gacaaggttg aacgctgtta tttcttcttg agtgcttttg 421 tggacacagc ccagagaaag gagcctgagg atgggaggct ggtgcagttt ttgcttatga 481 accctgcaaa tgatggtggc caatgggata tgcttgttaa tattgttgaa aaatatggtg 541 ttatccctaa gaaatgcttc cctgaatctt atacaacaga ggcaaccaga aggatgaatg 601 atattctgaa tcacaagatg agagaattct gtatacgact gcggaacctg gtacacagtg 661 gagcaaccaa aggagaaatc tcggccacac aggacgtcat gatggaggag atattccgag 721 tggtgtgcat ctgtttgggt aatccaccag agacattcac ctgggaatat cgagacaaag 781 ataaaaatta tcagaaaatt ggccccataa cacccttgga gttttacagg gaacatgtCA 841 AGCCACTCTT CAATATGGAA GATAAGATTT GTTTAGTGAA TGACCCTAGG CCCCAGCACA 901 AGTACAACAA ACTTTACACA GTGGAATACT TAAGCAATAT GGTTGGAGGG AGAAAAACTC 961 TATACAACAA CCAGCCCATT GACTTCCTGA AAAAGATGGT TGCTGCCTCC ATCAAAGATG 1021 GAGAGGCTGT GTGGTTTGGC TGTGATGTTG GAAAACACTT CAATAGCAAG CTGGGCCTCA 1081 GTGACATGAA TCTCTATGAC CATGAGTTAG TGTTTGGTGT CTCCTTGAAG AACATGAATA 1141 AAGCGGAGAG GCTGACTTTT GGTGAGTCAC TTATGACCCA CGCCATGACC TTCACTGCTG 1201 TCTCAGAGAA GGATGATCAG GATGGTGCTT TCACAAAATG GAGAGTGGAG AATTCATGGG 1261 GTGAAGACCA TGGCCACAAA GGTTACCTGT GCATGACAGA TGAGTGGTTC TCTGAGTATG 1321 TCTACGAAGT GGTGGTGGAC AGGAAGCATG TCCCTGAAGA GGTGCTAGCT GTGTTAGAGC 1381 AGGAACCCAT TATCCTGCCA GCATGGGACC CCATGGGAGC TTTGGCTGAG TGCCCAACTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGTGGAAA GGACGAACAG TTGATATCCT CAAGATTTTC ACGCGTTAAG TCgacaatca 1621 acctctggat tacaaaattt gtgaaagatt