Construct: ORF TRCN0000474964
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005790.1_s317c1
- Derived from:
- ccsbBroadEn_02639
- DNA Barcode:
- GATGAGCTTCCTTTAGACCCCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSTL1 (11167)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474964
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11167 | FSTL1 | follistatin like 1 | NM_007085.5 | 100% | 100% | |
| 2 | human | 11167 | FSTL1 | follistatin like 1 | XM_024453327.1 | 71.9% | 63% | (many diffs) |
| 3 | mouse | 14314 | Fstl1 | follistatin-like 1 | NM_008047.5 | 88.9% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 990
- ORF length:
- 924
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gaaacgctgg ctcgcgctcg cgctcgcgct ggtggcggtc gcctgggtcc 121 gcgccgagga agagctaagg agcaaatcca agatctgtgc caatgtgttt tgtggagccg 181 gccgggaatg tgcagtcaca gagaaagggg aacccacctg tctctgcatt gagcaatgca 241 aacctcacaa gaggcctgtg tgtggcagta atggcaagac ctacctcaac cactgtgaac 301 tgcatcgaga tgcctgcctc actggatcca aaatccaggt tgattacgat ggacactgca 361 aagagaagaa atccgtaagt ccatctgcca gcccagttgt ttgctatcag tccaaccgtg 421 atgagctccg acgtcgcatc atccagtggc tggaagctga gatcattcca gatggctggt 481 tctctaaagg cagcaactac agtgaaatcc tagacaagta ttttaagaac tttgataatg 541 gtgattctcg cctggactcc agtgaattcc TGAAGTTTGT GGAACAGAAT GAAACTGCCA 601 TCAATATTAC AACGTATCCA GACCAGGAGA ACAACAAGTT GCTTAGGGGA CTCTGTGTTG 661 ATGCTCTCAT TGAACTGTCT GATGAAAATG CTGATTGGAA ACTCAGCTTC CAAGAGTTTC 721 TCAAGTGCCT CAACCCATCT TTCAACCCTC CTGAGAAGAA GTGTGCCCTG GAGGATGAAA 781 CGTATGCAGA TGGAGCTGAG ACCGAGGTGG ACTGTAACCG CTGTGTCTGT GCCTGTGGAA 841 ATTGGGTCTG TACAGCCATG ACCTGTGACG GAAAGAATCA GAAGGGGGCC CAGACCCAGA 901 CAGAGGAGGA GATGACCAGA TATGTCCAGG AGCTCCAAAA GCATCAGGAA ACAGCTGAAA 961 AGACCAAGAG AGTGAGCACC AAAGAGATCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1021 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1081 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGATGA 1141 GCTTCCTTTA GACCCCTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1201 tgaaagatt