Construct: ORF TRCN0000474985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017265.1_s317c1
- Derived from:
- ccsbBroadEn_07306
- DNA Barcode:
- TCCCCTCCTGGTGATATTTGCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF10A (8797)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8797 | TNFRSF10A | TNF receptor superfamily me... | NM_003844.4 | 99.9% | 99.7% | 1322G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccaccacca gctagagtac atctaggtgc gttcctggca gtgactccga 121 atcccgggag cgcagcgagt gggacagagg cagccgcggc cacacccagc aaagtgtggg 181 gctcttccgc ggggaggatt gaaccacgag gcgggggccg aggagcgctc cctacctcca 241 tgggacagca cggacccagt gcccgggccc gggcagggcg cgccccagga cccaggccgg 301 cgcgggaagc cagccctcgg ctccgggtcc acaagacctt caagtttgtc gtcgtcgggg 361 tcctgctgca ggtcgtacct agctcagctg caaccatcaa acttcatgat caatcaattg 421 gcacacagca atgggaacat agccctttgg gagagttgtg tccaccagga tctcatagat 481 cagaacatcc tggagcctgt aaccggtgca cagagggtgt gggttacacc aatgcttcca 541 acaatttgtt tgcttgcctc ccatgtacag cttgtaaatc agatgaagaa gagagaagtc 601 cctgcaccac gaccaggaac acagcatgtc agtgcaaacc aggaactttc cggaatgaca 661 attctgctga gatgtgccgg aagtgcagca gagggtgccc cagagggatg gtcaaggtca 721 aggattgtac gccctggagt gacatcgagt gtgtccacaa agaatcaggc aatggacata 781 atatatgggt gattttggtt gtgactttgg ttgttccgtt gctgttggtg gctgtgctga 841 ttgtctgttg ttgcatcggc tcaggttgtg gaggggaccc caagtgcatg gacagggtgt 901 gtttctggcg cttgggtctc ctacgagggc ctggggctga ggacaatgct cacaacgaga 961 ttctgagcaa cgcagactcg ctgtccactt tcgtctctga gcagcaaatg gaaagccagg 1021 agccggcaga tttgacaggt gtcactgtac agtccccagg ggaggcacag tgtctgctgg 1081 gaccggcaga agctgaaggg tctcagagga ggaggctgct ggttccagca aatggtgctg 1141 accccactga gactctgatg ctgttctttg acaagtttgc aaacatcgtg ccctttgact 1201 cctgggacca gctcatgagg cagctggacc tcacgaaaaa tgagatcGAT GTGGTCAGAG 1261 CTGGTACAGC AGGCCCAGGG GATGCCTTGT ATGCAATGCT GATGAAATGG GTCAACAAAA 1321 CTGGACGGAA CGCCTCGATC CACACCCTGC TGGATGCCTT GGAGAGGATG GAAGAGAGAC 1381 ATGCAAAAGA GAAGATTCAG GACCTCTTGG TGGACTCTGG AAAGTTCATC TACTTAGAAG 1441 ATGGCACAGG CTCTGCCGTG TCCTTGGAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCCCC 1621 TCCTGGTGAT ATTTGCGACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt