Construct: ORF TRCN0000475019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005584.1_s317c1
- Derived from:
- ccsbBroadEn_12954
- DNA Barcode:
- CGTGTAGTGATTACCACAGGCTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AP5B1 (91056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475019
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91056 | AP5B1 | adaptor related protein com... | NM_138368.5 | 40.4% | 29.3% | 1_978del;1739_2328del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 846
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttgcgctc aaggcggcct ttggtgaggc cttgttcaca gcccaggatg 121 aagcgttgct gctccgccgg ctcaccttgg ctgcccagca ccctgctctg cctccgccca 181 cccatctctt ttaccttcac tgcgtcctga gcttccctga gaactggccg ctgggccctg 241 aaggtgagga ggctgcccca ctgctgctag ggccccagct atgccgtggt ctcctgccca 301 gtctcctgca tgacccaatg gccctcctgg cccgcctgca tttactgtgc ctgctctgtg 361 ccgaggagga agaagaggag aaaggccagc ttccaagccc acggcactac ctggaagagc 421 tgctggctgg cttgcggcag cgggcagccc tggatggggg cccccgggcc ttggccactc 481 tctgcttcca ggcctcgtat ctggtggcct gctgcctggc tgggcaacct acggtgctga 541 cccccttgat ccacggactg gcccagctgt accaagcccg gcccatgctg gctccccact 601 ttgtggacct cttggatcag gtggactctg agctgaggga gccccTGAAG GTGGTGTTGC 661 GGCAGGTGGT GGTGTCCAGG CCGGGCAGGG ATGAAGCTCT TTGCTGGCAC CTGCAAATGC 721 TGGCAAAGGT GGCAGATGGA GATGCCCAGA GTGCTACCCT CAACTTTCTA CAGGCCGCGG 781 CTGCCCACTG CACGAACTGG GACCTACAGC AGGGCCTGCT GCGGGTCTGC CGGGCTGGGC 841 TTCTTTGAGG AGCTCTGGGA TTCCTGCCTG CCAGAGGGTG CTGAGAGTCG TGTGTGGTGT 901 CCACTTGGGC CACAGGGCCT GGAGGGCTTG GTGTCCCGCC ACCTGGAGCC TTTTGTGGTG 961 GTGGCCCAGC CTCCTACCAG CTACTGTGTA GCAATCCACC TGCCCCCGGA CTCAAAGCTG 1021 CTGCTGCGGC TGGAGGCGGC CCTGGCAGAT GGAGTGCCTG TGGCCCTGCG GACCGATGAC 1081 TGGGCCGTGC TGCCCCTGGC GGGGGACTAC CTCCGTGGGC TGGCGGCTGC TGTCTTGCCA 1141 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 1201 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 1261 ATATCTTGTG GAAAGGACGA CGTGTAGTGA TTACCACAGG CTTCACGCGT TAAGTCgaca 1321 atcaacctct ggattacaaa atttgtgaaa gatt