Construct: ORF TRCN0000475081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004639.1_s317c1
- Derived from:
- ccsbBroadEn_04298
- DNA Barcode:
- AACCTGCTGTATATCTTTTCAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBP5 (83758)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475081
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83758 | RBP5 | retinol binding protein 5 | NM_031491.4 | 100% | 100% | |
| 2 | human | 83758 | RBP5 | retinol binding protein 5 | XM_017020002.1 | 88.7% | 79.8% | (many diffs) |
| 3 | human | 83758 | RBP5 | retinol binding protein 5 | NM_001329454.2 | 63.7% | 63.7% | 0_1ins147 |
| 4 | human | 83758 | RBP5 | retinol binding protein 5 | XR_001748882.1 | 56.2% | 1_167del;573_720del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcccaacctc actggctact accgctttgt ctcgcagaag aacatggagg 121 actacctgca agccctaaac atcagcttGG CTGTGCGGAA GATCGCGCTG CTGCTGAAGC 181 CGGACAAGGA GATCGAACAC CAGGGCAACC ACATGACGGT GAGGACGCTC AGCACCTTCC 241 GAAACTACAC TGTGCAGTTT GATGTGGGAG TGGAGTTTGA GGAGGACCTC AGGAGCGTGG 301 ACGGACGAAA ATGCCAGACC ATAGTAACCT GGGAGGAGGA GCACCTGGTG TGTGTGCAGA 361 AAGGGGAGGT CCCCAACCGG GGCTGGAGAC ACTGGCTGGA GGGAGAGATG CTGTATCTGG 421 AACTGACTGC AAGGGATGCA GTGTGCGAGC AGGTCTTCAG GAAGGTCAGA TACCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 601 GACGAAACCT GCTGTATATC TTTTCAGGAA CGCGTTAAGT Cgacaatcaa cctctggatt 661 acaaaatttg tgaaagatt