Construct: ORF TRCN0000475178

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015365.1_s317c1
Derived from:
ccsbBroadEn_06716
DNA Barcode:
GATTGGATGAATGGCCCCCCTACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFKM (5213)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475178
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5213 PFKM phosphofructokinase, muscle NM_000289.6 99.9% 100% 2334T>G
2 human 5213 PFKM phosphofructokinase, muscle NM_001166687.1 99.9% 100% 2334T>G
3 human 5213 PFKM phosphofructokinase, muscle NM_001166688.1 99.9% 100% 2334T>G
4 human 5213 PFKM phosphofructokinase, muscle NM_001354742.1 99.9% 100% 2334T>G
5 human 5213 PFKM phosphofructokinase, muscle NM_001354743.1 99.9% 100% 2334T>G
6 human 5213 PFKM phosphofructokinase, muscle NM_001354744.1 99.9% 100% 2334T>G
7 human 5213 PFKM phosphofructokinase, muscle XM_024449022.1 99.9% 100% 2334T>G
8 human 5213 PFKM phosphofructokinase, muscle NM_001354741.1 98.9% 98.9% 1_24del;2358T>G
9 human 5213 PFKM phosphofructokinase, muscle NM_001354745.1 96.2% 96.2% 0_1ins87;2247T>G
10 human 5213 PFKM phosphofructokinase, muscle NM_001363619.1 95.9% 96% 843_844ins93;2241T>G
11 human 5213 PFKM phosphofructokinase, muscle XM_024449021.1 95.8% 95.9% 1_99del;2433T>G
12 human 5213 PFKM phosphofructokinase, muscle NM_001354746.1 94.5% 94.6% 934_935ins126;2208T>G
13 human 5213 PFKM phosphofructokinase, muscle NM_001354740.1 94.1% 94.2% 1_144del;2478T>G
14 human 5213 PFKM phosphofructokinase, muscle NM_001354747.1 93.4% 93.2% (many diffs)
15 human 5213 PFKM phosphofructokinase, muscle NM_001354748.1 93.4% 93.2% (many diffs)
16 human 5213 PFKM phosphofructokinase, muscle NM_001166686.2 91.6% 91.6% 1_213del;2547T>G
17 human 5213 PFKM phosphofructokinase, muscle NM_001354737.1 91.6% 91.6% 1_213del;2547T>G
18 human 5213 PFKM phosphofructokinase, muscle NM_001354738.1 91.6% 91.6% 1_213del;2547T>G
19 human 5213 PFKM phosphofructokinase, muscle NM_001354739.1 91.6% 91.6% 1_213del;2547T>G
20 human 5213 PFKM phosphofructokinase, muscle XM_005268979.1 91.6% 91.6% 1_213del;2547T>G
21 human 5213 PFKM phosphofructokinase, muscle XM_024449020.1 91.2% 91.3% 1_222del;2556T>G
22 human 5213 PFKM phosphofructokinase, muscle NM_001354735.1 88.2% 88.3% 1_309del;2643T>G
23 human 5213 PFKM phosphofructokinase, muscle NM_001354736.1 88.2% 88.3% 1_309del;2643T>G
24 human 5213 PFKM phosphofructokinase, muscle XM_005268974.1 88.2% 88.3% 1_309del;2643T>G
25 human 5213 PFKM phosphofructokinase, muscle XM_005268976.3 88.2% 88.3% 1_309del;2643T>G
26 human 5213 PFKM phosphofructokinase, muscle XM_017019469.1 87.9% 88% 1_213del;1056_1057ins93;2454T>G
27 human 5213 PFKM phosphofructokinase, muscle XM_011538487.1 84.7% 84.8% 1_309del;1152_1153ins93;2550T>G
28 human 5213 PFKM phosphofructokinase, muscle NR_148958.1 69.2% (many diffs)
29 human 5213 PFKM phosphofructokinase, muscle NR_148954.1 67.2% (many diffs)
30 human 5213 PFKM phosphofructokinase, muscle NR_148959.1 67% (many diffs)
31 human 5213 PFKM phosphofructokinase, muscle NR_148956.1 65.1% (many diffs)
32 human 5213 PFKM phosphofructokinase, muscle NR_148957.1 64.4% (many diffs)
33 human 5213 PFKM phosphofructokinase, muscle NR_148955.1 56.8% (many diffs)
34 mouse 18642 Pfkm phosphofructokinase, muscle NM_001163487.1 90.1% 97.8% (many diffs)
35 mouse 18642 Pfkm phosphofructokinase, muscle NM_001163488.1 90.1% 97.8% (many diffs)
36 mouse 18642 Pfkm phosphofructokinase, muscle NM_021514.4 90.1% 97.8% (many diffs)
37 mouse 18642 Pfkm phosphofructokinase, muscle XM_006520602.2 90.1% 97.8% (many diffs)
38 mouse 18642 Pfkm phosphofructokinase, muscle XM_006520601.3 82.7% 89.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2406
ORF length:
2340
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac ccatgaagag caccatgcag ccaaaaccct ggggattggc aaagccattg
121ctgtcttaac ctctggtgga gatgcccaag gtatgaatgc tgctgtcagg gctgtggttc
181gagttggtat cttcaccggt gcccgtgtct tctttgtcca tgagggttat caaggcctgg
241tggatggtgg agatcacatc aaggaagcca cctgggagag cgtttcgatg atgcttcagc
301tgggaggcac ggtgattgga agtgcccggt gcaaggactt tcgggaacga gaaggacgac
361tccgagctgc ctacaacctg gtgaagcgtg ggatcaccaa tctctgtgtc attgggggtg
421atggcagcct cactggggct gacaccttcc gttctgagtg gagtgacttg ttgagtgacc
481tccagaaagc aggtaagatc acagatgagg aggctacgaa gtccagctac ctgaacattg
541tgggcctggt tgggtcaatt gacaatgact tctgtggcac cgatatgacc attggcactg
601actctgccct gcatcggatc atggaaattg tagatgccat cactaccact gcccagagcc
661accagaggac atttgtgtta gaagtaatgg gccgccactg tggatacctg gcccttgtca
721cctctctgtc ctgtggggcc gactgggttt ttattcctga atgtccacca gatgacgact
781gggaggaaca cctttgtcgc cgactcagcg agacaaggac ccgtggttct cgtctcaaca
841tcatcattgt ggctgagggt gcaattgaca agaatggaaa accaatcacc tcagaagaca
901tcaagaatct ggtggttaag cgtctgggat atgacacccg ggttactgtc ttggggcatg
961tgcagagggg tgggacgcca tcagcctttg acagaattct gggcagcagg atgggtgtgg
1021aagcagtgat ggcacttttg gaggggaccc cagatacccc agcctgtgta gtgagcctct
1081ctggtaacca ggctgtgcgc ctgcccctca tggaatgtgt ccaggtgacc aaagatgtga
1141ccaaggccat ggatgagaag aaatttgacg aagccctgaa gctgagaggc cggagcttca
1201tgaacaactg ggaggtgtac aagcttctag ctcatgtcag acccccggta tctaagagtg
1261gttcgcacac agtggctgtg atgaacgtgg gggctccggc tgcaggcatg aatgctgctg
1321ttcgctccac tgtgaggatt ggccttatcc agggcaaccg agtgctcgtt gtccatgatg
1381gtttcgaggg cctggccaag gggcagatag aggaagctgg ctggagctat gttgggggct
1441ggactggcca aggtggctct aaacttggga ctaaaaggac tctacccaag aagagctttg
1501aacagatcag tgccaatata actaagttta acattcaggg ccttgtcatc attgggggct
1561ttgaggctta cacagggggc ctggaactga tggagggcag gaagcagttt gatgagctct
1621gcatcccatt tgtggtcatt cctgctacag tctccaacaa tgtccctggc tcagacttca
1681gcgttggggc tgacacagca ctcaatacta tctgcacaac ctgtgaccgc atcaagcagt
1741cagcagctgg caccaagcgt cgggtgttta tcattgagac tatgggtggc tactgtggct
1801acctggctac catggctgga ctggcagctg gggccgatgc tgcctacatt tttgaggagc
1861ccttcaccat tcgagacctg caggcaaatg ttgaacatct ggtgcaaaag atgaaaacaa
1921ctgtgaaaag gggcttggtg ttaaggaatg aaaagtgcaa tgagaactat accactgact
1981tcattttcaa cctgtactct gaggagggga agggcaTCTT CGACAGCAGG AAGAATGTGC
2041TTGGTCACAT GCAGCAGGGT GGGAGCCCAA CCCCATTTGA TAGGAATTTT GCCACTAAGA
2101TGGGCGCCAA GGCTATGAAC TGGATGTCTG GGAAAATCAA AGAGAGTTAC CGTAATGGGC
2161GGATCTTTGC CAATACTCCA GATTCGGGCT GTGTTCTGGG GATGCGTAAG AGGGCTCTGG
2221TCTTCCAACC AGTGGCTGAG CTGAAGGACC AGACAGATTT TGAGCATCGA ATCCCCAAGG
2281AACAGTGGTG GCTGAAACTG AGGCCCATCC TCAAAATCCT AGCCAAGTAC GAGATTGACT
2341TGGACACTTC AGACCATGCC CACCTGGAGC ACATCACCCG GAAGCGGTCC GGGGAAGCGG
2401CCGTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2461TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2521CTTGGCTTTA TATATCTTGT GGAAAGGACG AGATTGGATG AATGGCCCCC CTACGACGCG
2581TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

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