Construct: ORF TRCN0000475182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014678.1_s317c1
- Derived from:
- ccsbBroadEn_11362
- DNA Barcode:
- TAGCCCCCGATCCTGTGGTAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB28 (9364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_001159601.1 | 46.5% | 43.6% | 262_391del;416_612del |
| 2 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_004249.4 | 43.1% | 40.4% | 262_391del;416_660del |
| 3 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_001017979.3 | 42.9% | 40.2% | 262_391del;416_663del |
| 4 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310590.1 | 45.5% | 44.5% | (many diffs) |
| 5 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310589.1 | 40% | 39% | (many diffs) |
| 6 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | XM_017320585.1 | 40% | 39% | (many diffs) |
| 7 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_027295.3 | 39.8% | 38.9% | (many diffs) |
| 8 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | XM_006503651.3 | 34.2% | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 351
- ORF length:
- 285
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC GGACTCTGAG GAGGAGAGCC AGGACCGGCA ACTGAAAATC GTCGTGCTGG 121 GGGACGGCGC CTCCGGGAAG ACCTCCTTAA CTACGTGTTT TGCTCAAGAA ACTTTTGGGA 181 AACAGTACAA ACAAACTATA GGACTGGATT TCTTTTTGAG AAGGATAACA TTGCCAGGAA 241 ACTTGAATGT TACCCTTCAA ATTTGGGATA TAGGAGGGCA GACAATAGGA GGCAAAATGT 301 TGGATAAATA TATCTATGGA GCACAGTTGA TTTGGAGCAT ATGCGAACAA TACCCAACTT 361 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 421 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 481 CTTGTGGAAA GGACGATAGC CCCCGATCCT GTGGTAATGA ACGCGTTAAG TCgacaatca 541 acctctggat tacaaaattt gtgaaagatt