Construct: ORF TRCN0000475235
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017711.1_s317c1
- Derived from:
- ccsbBroadEn_05048
- DNA Barcode:
- GTTGGGTCCCCACCTTATAAGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC140 (151278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475235
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151278 | CCDC140 | coiled-coil domain containi... | NR_161172.1 | 28.7% | 1_384del;874_1699del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 558
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagatgaa tgttcaaacc cagatctcct cgctgagcct ggaagctcgc 121 caccgtggga tcacgggaac cagaggcaag aggctgcgaa tgagtccaac acccgcgTTC 181 CTCGAGTTTT AAAAGCACAC CTGGGGCCAG AGACTGCACA GCCAACTAAA CGGAGTAAAC 241 GCAACAGGTG GAGGAGGCAG AGTTGTCAGG GGCCAAGCCC GGCGCGATCT GGCCAGTTCT 301 TGGGGAGCGC GGACCTGGGA CTGCAGAGAG GCGTTTTGAA GAGTGCTGCG CGCACCTGCC 361 TCTCGGAGAT ATCCAACTCC ACCCGGGCGT CTCCTGAGAG CGCACAGTCC ACAGACCCCG 421 GGAGGGCTGC CCGCCCTAGG ACACGTACTC TCCCGACCCC TCACTCTTTC AAAATTGGGG 481 AAGAGGCGGA GGAGATGAAA AAGAAGAAAG AGAGAAAGAG AAGAAAAGAG AGAAAGAAGG 541 AAAGAAATTT TAAAAAATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTGGGT CCCCACCTTA 721 TAAGGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt