Construct: ORF TRCN0000475236
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008038.1_s317c1
- Derived from:
- ccsbBroadEn_03175
- DNA Barcode:
- ATGCACCCATGATTTAGCCCCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS16 (51021)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475236
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51021 | MRPS16 | mitochondrial ribosomal pro... | NM_016065.4 | 100% | 100% | |
| 2 | mouse | 66242 | Mrps16 | mitochondrial ribosomal pro... | NM_025440.2 | 83.9% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 502
- ORF length:
- 411
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAAGTTGGC ATGGTCCACC TCACTACTCT CCTCTGCAAG 121 GCCTACCGTG GGGGCCACTT AACCATCCGC CTTGCCCTGG GTGGCTGCAC CAATCGGCCG 181 TTCTACCGCA TTGTGGCTGC TCACAACAAG TGTCCCAGGG ATGGCCGTTT CGTAGAGCAG 241 CTGGGCTCCT ATGATCCATT GCCCAACAGT CATGGAGAAA AACTCGTTGC CCTCAACCTA 301 GACAGGATCC GTCATTGGAT TGGCTGCGGG GCCCACCTCT CTAAGCCTAT GGAAAAGCTT 361 CTGGGTCTTG CTGGCTTTTT CCCTCTGCAT CCTATGATGA TCACAAATGC TGAGAGACTG 421 CGAAGGAAAC GGGCACGTGA AGTCCTGTTA GCTTCTCAGA AAACAGATGC AGAAGCTACA 481 GATACAGAGG CTACAGAAAC ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 541 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 601 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATG CACCCATGAT 661 TTAGCCCCGG CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 721 t