Construct: ORF TRCN0000475242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008031.1_s317c1
- Derived from:
- ccsbBroadEn_03122
- DNA Barcode:
- ATGATCTTTCCAGTAAGTGCGCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHOD (29984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29984 | RHOD | ras homolog family member D | NM_014578.4 | 100% | 100% | |
| 2 | human | 29984 | RHOD | ras homolog family member D | NM_001300886.2 | 68.5% | 68.5% | 131_132ins198 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggcggcccag gccgcgggtg aggaggcgcc accaggcgtg cggtccgtca 121 aggtggtcct ggtgggcgac ggcggctgcg ggaagacgtc gctgctgatg gtcttcgccg 181 atggggcctt ccccgagagc tacaccccca cggtgtttga gcggtacatg gtcaaccTGC 241 AAGTGAAAGG CAAACCTGTG CACCTCCACA TCTGGGACAC AGCAGGGCAA GATGACTATG 301 ACCGCCTGCG GCCCCTGTTC TACCCTGACG CCAGCGTCCT GCTGCTTTGC TTCGATGTCA 361 CCAGCCCGAA CAGCTTTGAC AACATCTTTA ACCGGTGGTA CCCAGAAGTG AATCATTTCT 421 GCAAGAAGGT ACCCATCATC GTCGTGGGCT GCAAGACTGA CCTGCGCAAG GACAAATCAC 481 TGGTGAACAA GCTCCGAAGA AACGGATTGG AGCCTGTGAC CTACCACAGG GGCCAGGAGA 541 TGGCGAGGTC CGTGGGCGCG GTGGCCTACC TCGAGTGCTC GGCTCGGCTC CATGACAACG 601 TCCACGCCGT CTTCCAGGAG GCCGCCGAGG TGGCCCTCAG CAGCCGCGGT CGCAACTTCT 661 GGCGGCGGAT TACCCAGGGC TTTTGCGTGG TGACCTGCCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 AATGATCTTT CCAGTAAGTG CGCATACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt