Construct: ORF TRCN0000475298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003899.1_s317c1
- Derived from:
- ccsbBroadEn_03301
- DNA Barcode:
- TGGCCTTAATTAAGTAAATTACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOL7 (51406)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51406 | NOL7 | nucleolar protein 7 | NM_001317724.1 | 100% | 100% | |
| 2 | human | 51406 | NOL7 | nucleolar protein 7 | NM_016167.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcagctccga ccgcgagcgt ctcgcgcccc ggcgtcggcg gaggcgatgg 121 tggacgaggg ccagctggcc tcggaggagg aggaggcgga gcacgggctg ttgctcgggc 181 agcccagcag cggcgcggcc gccgagcccc tggaggaaga cgaggaaggg gacgatgagt 241 ttgacgatga ggccccggag gagctgactt tcgccagcgc ccaggcggaa gcgagagaag 301 aggagcggcg agtgcgggag accgtgcgca gggataaaac gctcctgaag gagaagagga 361 agcgacgcga ggagctgttc atcgaacaga agaaaagaaa actccttcca gacactattt 421 tggagaagtt aaccacagct tcacagacta acatcaagaa atcgccagga aaggtgaaaG 481 AAGTTAATTT GCAAAAGAAA AATGAAGACT GTGAAAAAGG AAATGACTCC AAGAAAGTTA 541 AAGTACAAAA AGTACAGTCT GTCAGCCAGA ATAAAAGCTA CTTGGCCGTA AGGCTAAAAG 601 ACCAAGATCT GAGAGATTCA AGGCAACAAG CAGCACAAGC CTTCATACAT AATTCATTAT 661 ATGGGCCAGG AACCAACAGG ACTACTGTAA ATAAGTTCCT GTCTCTTGCC AACAAGAGGT 721 TACCAGTGAA AAGAGCTGCT GTCCAGTTTT TGAATAATGC TTGGGGAATC CAAAAAAAAC 781 AAAATGCCAA GAGGTTTAAA AGACGGTGGA TGGTCAGAAA GATGAAAACT AAGAAGTACC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 961 ATATATCTTG TGGAAAGGAC GATGGCCTTA ATTAAGTAAA TTACCAACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt