Construct: ORF TRCN0000475316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002212.1_s317c1
- Derived from:
- BRDN0000396060
- DNA Barcode:
- TCTTCCGCCGCGACTCCTAGCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX51 (317781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 317781 | DDX51 | DEAD-box helicase 51 | NM_175066.4 | 99.4% | 98.3% | (many diffs) |
| 2 | human | 317781 | DDX51 | DEAD-box helicase 51 | XM_011538256.1 | 77.7% | 76.4% | (many diffs) |
| 3 | human | 317781 | DDX51 | DEAD-box helicase 51 | XR_001748676.1 | 73.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2064
- ORF length:
- 1998
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgttctac gtcgcgcggt acccgggccc cgatgcggca gctgcggcgg 121 ggccggaggg cgcggaggcc ggggcgcacg gcagggcccg cgcgctgctc gagcggctgc 181 agagccaggc ccgcgaacgg cagcagcagc gggagcccgc gcagaccgag gcggctgcat 241 cgaccgagcc ggcgaccagg aggcgacggc ggccccggcg gcggcggcgg gtgaacgacg 301 cggagccggg gagcccggag gcgccgcagg gaaagcgacg gaaggcggac ggcgaggacg 361 cgggcgcaga aagcaacgag gaggcgccag gggagcccag cgcagggagc agcgaggagg 421 cgcaaggggg gcccagcgca gggagcagcg aggaggcgcc gggggtgcgc agcaccagcg 481 ccagcgccga ggcggcccca gatggaccgg ccctggagga ggcggccgga cccctggtcc 541 ccggcctggt gctggggggg ttcgggaaga ggaaggcgcc gaaggtcaag cctttcctgc 601 caaggtggct ggctgagcct aactgtgtca gaaggaatgt caccgaagac ctggttccta 661 tcgaggacat ccctgacgtc catcctgacc tgcagaagca gctgcgggca cacggcatct 721 cgtcctactt tccagtccag gcagctgtga ttcctgccct cctggagagc gcagcctgtg 781 ggtttctggt gggcagaggt ggctaccggc ttagcgacct ctgtgtttct gccccaacag 841 gcagtgggaa gacactggcc ttcgtcatcc ctgtggtgca ggccctgctt tcgagagtgg 901 tctgccacat ccgtgccctg gttgtgctgc ccaccaagga gctggcccgg caggtgagca 961 aagttttcaa catctacaca gatgccacac ctctgagagt ctccctggtt acgggacaga 1021 agtctctggc caaggagcag gagagcctcg tccagaaaac agctgatggg taccgctgct 1081 tggctgacat cgtggtagcc acccccggcc gcctggtgga ccacatcgac cagaccccag 1141 gattcagcct ccagcagctc cgcttcctga ttatcgacga ggctgaccgg atgattgaca 1201 gcatgcatca gtcctggctg ccgcgggtgg tggcggccgc cttccagagc gaggaccccg 1261 cggacccctg tgccctgctc aagcgaaggc aggcccaggc tgtgacagcc gccagcacct 1321 gctgtcccca gatgcccctg cagaagctgc tcttctcagc tactctgacc cagaaccctg 1381 aaaagctgca gcagctgggc ctccaccagc cccggctttt ctccacaggg ctagcacaca 1441 ggggcctgga ccatacagat ggggacgggg attcggggaa gtatgccttt cctgttgggc 1501 tcacgcacca ctacgtgccc tgcagcctca gctctaagcc gctggtcgtc ctgcacctgg 1561 tcctggagat gggcttctcg agggttctct gcttcactaa ctcccgagag aactcccaca 1621 ggctcttcct gctggtgcaa gcttttgggg gtgtggacgt ggctgagttc tcctcgcgct 1681 acgggcctgg ccagaggagg atgatcctga agcagtttga acaggggaag atccagctgc 1741 tcatcagcac ggacgccacc gcgcgaggca tcgacgtgca gggtgtggag ctggtggtga 1801 acTACGACGC CCCCCAGTAC CTGAGAACCT ACGTGCACCG GGTTGGGAGG ACAGCTCGCG 1861 CTGGGAAAAC TGGACAGGCC TTCACACTGC TCCTGAAAGT GCAGGAGAGG AGATTCCTCC 1921 GAATGCTAAC TGAAGCTGGG GCACCTGAGT TGCAGCGGCA CGAGCTCTCC AGCAAGCTGC 1981 TGCAGCCGCT GGTTCCTCGG TACGAGGAGG CCCTGTCCAA GCTGGAGGAG TCTGTCAAGG 2041 AAGAGCGCAA GCAGAGGGCG GCCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2101 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2161 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CTTCCGCCGC 2221 GACTCCTAGC CGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2281 att