Construct: ORF TRCN0000475337
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013678.1_s317c1
- Derived from:
- ccsbBroadEn_12380
- DNA Barcode:
- ACGTTGCATCCCAAGCTTTGAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC146 (57639)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475337
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57639 | CCDC146 | coiled-coil domain containi... | NM_020879.3 | 31.1% | 30.9% | 1_1971del;1974A>G;2357A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tatccaagag aagatgaaac taaatggaga aattgaaata catctactgg 121 aagaaaagat ccaattcctg aaaatgaaga ttgctgagaa gcaaagacaa atttgtgtga 181 cccagaaatt actgccagcc aagaggtccc tggatgccga cctagctgtg ctccaaattc 241 agttttcaca gtgtacagac agaattaaag acctggagaa acagttcgta aagcctgatg 301 gtgagaatag agctcgcttc cttccaggga aagatctgac cgaaaaagaa atgatccaaa 361 aattagacaa gctggaacta caactggcca agaaggagga gaagctgctg gagaaggatt 421 tcatctatga gcaggtctcc aggctcacag gcaggctctg cagcaaaact cagggctgca 481 agcaggacac actgctctta gccaagaaga tgaatggcta tcaaagaagg atcaaaaatg 541 caactgagaa aatgatggct cttgttgcTG AGCTGTCCAT GAAACAAGCC CTAACCATTG 601 AACTCCAAAA GGAAGTCAGG GAGAAAGAAG ACTTCATCTT CACTTGCAAT TCCAGGATAG 661 AAAAAGGTCT GCCACTCAAT AAGGAAATTG AGAAAGAATG GTTGAAAGTC CTTCGAGATG 721 AAGAAATGCA CGCCTTGGCC ATCGCTGAAA AGTCTCAGGA GTTCTTGGAA GCAGATAATC 781 GCCAGCTGCC CAATGGTGTT TACACAACTG CAGAGCAGCG TCCGAATGCC TACATCCCAG 841 AAGCAGATGC CACTCTTCCT TTGCCAAAAC CTTATGGTGC TTTGGCTCCT TTTAAACCCA 901 GTGAACCTGG AGCCAATATG AGGCACATAA GGAAACCTGT TATAAAGCCA GTTGAAATCT 961 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAACGTT GCATCCCAAG CTTTGAGAGA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt