Construct: ORF TRCN0000475359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016146.1_s317c1
- Derived from:
- ccsbBroadEn_13254
- DNA Barcode:
- TCTTGGAAGTCTGCGCGTGAGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYHIN1 (149628)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475359
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_005244930.2 | 100% | 100% | |
| 2 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_011509243.2 | 46.9% | 42.2% | (many diffs) |
| 3 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_198929.5 | 46.5% | 41.8% | (many diffs) |
| 4 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_017000463.1 | 46.4% | 41.7% | (many diffs) |
| 5 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_198930.4 | 44.6% | 40% | (many diffs) |
| 6 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_152501.5 | 43.6% | 39.3% | (many diffs) |
| 7 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | XM_011509242.2 | 43.6% | 39.3% | (many diffs) |
| 8 | human | 149628 | PYHIN1 | pyrin and HIN domain family... | NM_198928.5 | 41.9% | 37.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 801
- ORF length:
- 735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aaataactac aagaaaattg ttctactgaa aggattagag gtcatcaatg 121 attatcattt tagaattgtt aagtccttac tgagtaacga tttaaaactt aatccaaaaa 181 tgaaagaaga gtatgacaaa attcagattg ctgacttgat ggaggaaaag ttcccaggtg 241 atgccggttt gggcaaacta atagaattct tcaaagaaat accaacactg ggagaccttg 301 ctgaaactct taaaagagaa aagttaaaag ttgcaaataa aattgaatcc attccagtca 361 aaggaataat cccatctaaa aagacgaaac agaaagaagt gtatcctgct acacctgcat 421 gcaccccaag caaccgtctc acagctaaag gagcagagga gactcttgga cctcagaaaa 481 gaaaaaaacc atctgaagaa gagactggaa ccaaaaggag taagatgtcc aaagagcaga 541 ctcggccttc ctgctctgca ggagccagca cgtccacagC CATGGGCCGT TCCCCACCTC 601 CCCAGACCTC ATCATCAGCT CCACCCAACA CTTCCTCAAC TGAGGCATAT TTGATGAACT 661 TGACATTATC TCTGACTGCA GGAAGTTTTC TGTCCTGTGC TGTTTGGGGA AGAGACAGAG 721 AACTGCGGAA TCTGGAACTT TCAGCAACAG ACTCACTGTC TACTGCCCCC ATCTATTATA 781 CACCCATTCC CTTTGCTCAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCTT GGAAGTCTGC 961 GCGTGAGATA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt