Construct: ORF TRCN0000475445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009085.1_s317c1
- Derived from:
- ccsbBroadEn_04085
- DNA Barcode:
- GAGGATCGCGGGGCACAATCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF128 (79589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79589 | RNF128 | ring finger protein 128 | NM_194463.2 | 100% | 100% | |
| 2 | human | 79589 | RNF128 | ring finger protein 128 | NM_024539.3 | 74.6% | 73.8% | (many diffs) |
| 3 | mouse | 66889 | Rnf128 | ring finger protein 128 | NM_023270.5 | 91.5% | 95.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1350
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gccgccgcct ggggccgggg tctcctgccg cggtggctgc ggcttttcca 121 gattgctggc atggtgcttc ctgctggccc tgagtccgca ggcacccggt tcccgggggg 181 ctgaagcagt gtggaccgcg tacctcaacg tgtcctggcg ggttccgcac acgggagtga 241 accgtacggt gtgggagctg agcgaggagg gcgtgtacgg ccaggactcg ccgctggagc 301 ctgtggctgg ggtcctggta ccgcccgacg ggcccggggc gcttaacgcc tgtaacccgc 361 acacgaattt cacggtgccc acggtttggg gaagcaccgt gcaagtctct tggttggccc 421 tcatccaacg cggcgggggc tgcaccttcg cagacaagat ccatctggct tatgagagag 481 gggcgtctgg agccgtcatc tttaacttcc ccgggacccg caatgaggtc atccccatgt 541 ctcacccggg tgcagtagac attgttgcaa tcatgatcgg caatctgaaa ggcacaaaaa 601 ttctgcaatc tattcaaaga ggcatacaag tgacaatggt catagaagta gggaaaaaac 661 atggcccttg ggtgaatcac tattcaattt ttttcgtttc tgtgtccttt tttattatta 721 cggcggcaac tgtgggctat tttatctttt attctgctcg aaggctacgg aatgcaagag 781 ctcaaagcag gaagcagagg caattaaagg cagatgctaa aaaagctatt ggaaggcttc 841 aactacgcac actgaaacaa ggagacaagg aaattggccc tgatggagat agttgtgctg 901 tgtgcattga attgtataaa ccaaatgatt tggtacgcaT CTTAACGTGC AACCATATTT 961 TCCATAAGAC ATGTGTTGAC CCATGGCTGT TAGAACACAG GACTTGCCCC ATGTGCAAAT 1021 GTGACATACT CAAAGCTTTG GGAATTGAGG TGGATGTTGA AGATGGATCA GTGTCTTTAC 1081 AAGTCCCTGT ATCCAATGAA ATATCTAATA GTGCCTCCTC CCATGAAGAG GATAATCGCA 1141 GCGAGACCGC ATCATCTGGA TATGCTTCAG TACAGGGAAC AGATGAACCG CCTCTGGAGG 1201 AACACGTGCA GTCAACAAAT GAAAGTCTAC AGCTGGTAAA CCATGAAGCA AATTCTGTGG 1261 CAGTGGATGT TATTCCTCAT GTTGACAACC CAACCTTTGA AGAAGACGAA ACTCCTAATC 1321 AAGAGACTGC TGTTCGAGAA ATTAAATCTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGGA 1501 TCGCGGGGCA CAATCACACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt