Construct: ORF TRCN0000475445

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009085.1_s317c1
Derived from:
ccsbBroadEn_04085
DNA Barcode:
GAGGATCGCGGGGCACAATCACAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF128 (79589)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475445
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79589 RNF128 ring finger protein 128 NM_194463.2 100% 100%
2 human 79589 RNF128 ring finger protein 128 NM_024539.3 74.6% 73.8% (many diffs)
3 mouse 66889 Rnf128 ring finger protein 128 NM_023270.5 91.5% 95.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1350
ORF length:
1284
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gccgccgcct ggggccgggg tctcctgccg cggtggctgc ggcttttcca
121gattgctggc atggtgcttc ctgctggccc tgagtccgca ggcacccggt tcccgggggg
181ctgaagcagt gtggaccgcg tacctcaacg tgtcctggcg ggttccgcac acgggagtga
241accgtacggt gtgggagctg agcgaggagg gcgtgtacgg ccaggactcg ccgctggagc
301ctgtggctgg ggtcctggta ccgcccgacg ggcccggggc gcttaacgcc tgtaacccgc
361acacgaattt cacggtgccc acggtttggg gaagcaccgt gcaagtctct tggttggccc
421tcatccaacg cggcgggggc tgcaccttcg cagacaagat ccatctggct tatgagagag
481gggcgtctgg agccgtcatc tttaacttcc ccgggacccg caatgaggtc atccccatgt
541ctcacccggg tgcagtagac attgttgcaa tcatgatcgg caatctgaaa ggcacaaaaa
601ttctgcaatc tattcaaaga ggcatacaag tgacaatggt catagaagta gggaaaaaac
661atggcccttg ggtgaatcac tattcaattt ttttcgtttc tgtgtccttt tttattatta
721cggcggcaac tgtgggctat tttatctttt attctgctcg aaggctacgg aatgcaagag
781ctcaaagcag gaagcagagg caattaaagg cagatgctaa aaaagctatt ggaaggcttc
841aactacgcac actgaaacaa ggagacaagg aaattggccc tgatggagat agttgtgctg
901tgtgcattga attgtataaa ccaaatgatt tggtacgcaT CTTAACGTGC AACCATATTT
961TCCATAAGAC ATGTGTTGAC CCATGGCTGT TAGAACACAG GACTTGCCCC ATGTGCAAAT
1021GTGACATACT CAAAGCTTTG GGAATTGAGG TGGATGTTGA AGATGGATCA GTGTCTTTAC
1081AAGTCCCTGT ATCCAATGAA ATATCTAATA GTGCCTCCTC CCATGAAGAG GATAATCGCA
1141GCGAGACCGC ATCATCTGGA TATGCTTCAG TACAGGGAAC AGATGAACCG CCTCTGGAGG
1201AACACGTGCA GTCAACAAAT GAAAGTCTAC AGCTGGTAAA CCATGAAGCA AATTCTGTGG
1261CAGTGGATGT TATTCCTCAT GTTGACAACC CAACCTTTGA AGAAGACGAA ACTCCTAATC
1321AAGAGACTGC TGTTCGAGAA ATTAAATCTT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1381TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1441CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGGA
1501TCGCGGGGCA CAATCACACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1561tgaaagatt

Download FASTA (ORF) (Full)