Construct: ORF TRCN0000475451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008670.1_s317c1
- Derived from:
- ccsbBroadEn_04967
- DNA Barcode:
- CTTCTCACTTCCGGTTCCGTTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNHD1 (144132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475451
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144132 | DNHD1 | dynein heavy chain domain 1 | NM_173589.4 | 100% | 100% | |
| 2 | human | 144132 | DNHD1 | dynein heavy chain domain 1 | NM_144666.2 | 12.5% | 12.5% | 1789G>T;1791_14259delinsC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1860
- ORF length:
- 1791
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcccggag gagaggaggg taggtttgtc ttctgatgag acatcatctg 121 attcccttaa gtcttggcac tccatatgtg tcttggacag caaagaacag cccttggcct 181 gccagcagaa acagaggcag ttcgtgaagc cagtgactga gtcagagcag cccacagtgc 241 tggaactcct gctagctgag ctccgaactc tgttctcagc tgtgttgcag gacagtagcc 301 ctgcagcttg gcgctatctt catgcagtac tgggtctact gcctccatat cgtgagttgc 361 tagttggcca ccttgatttg ctgcccttcc tggagcagct gtactgctgg gcaccctggg 421 tccaaaccca cctccatctg gacctgctag gtgccattgt ccaggccttt cctccagaca 481 gctctttgtt agacagtgct tcccatgctg actgctgtcc ccagaagcgg aggctccatc 541 acaggccccc atgcccagct tgcccttttg tgcaggccca gtggagcagg cagcaagtaa 601 aggaggagct ggccacctgg ctgcgaccat tgacactgcc tgagctacag cgctgcctgg 661 gcattgttgg tgctcaggtg gccctagaag aggctgtgtg gctggatgga cttagtctcc 721 ttcccttggc actggcagcg gacatccctg tacggtatga aagcagtgac actgacaatg 781 cagaggtgga gcctgttgga agaaaagaga ccaggtctca gcttgactat gaagttccca 841 gggaaaaggc cttccaaaag agcagcaccg gcttttcacc tgagacttcc ttcctggata 901 gccaggtgat gactgctctg aagatggaga gatacctgaa gaagatccac ttcctctatc 961 tcaatgtggc tcccagccgg tactttaggc cttacagcct gatggtggtg ccacccgaca 1021 aggtgaatcc cgagcactac atcttctctc cctttgggat cttgcatgta catcctgtgg 1081 aaggtagcga gacgatgaca ctgggtacct ggcaccatca ctgtgttctc tggcagcagc 1141 tccagttcat tccattcttt aagtattgcc tcttacgcaa gtcctttacc tgttggaaga 1201 agaatgtgag attacagggg ctgcatcgac tccagaaatt cctagagaat catctgctct 1261 tggctgtgcc ccactttgga gctgggctac tccatattag caggcttctg caggagctac 1321 actctgtgtc ctggctaccc caggaactgg atcggtgcta tgagctgctg gacctgcaga 1381 cggctctagc cgaggagaag cataaggctc tacggctgct ccatcgttgc ctaaacctct 1441 gcacatccat tcttcgactg gttcacgagg acacatacca catgcaacag tgccTACAGG 1501 AGCGAGTACA AAACTGTGAC AGGATCAGGA CAGGCCAAGG CTCCATATAC CTTCAGAGGG 1561 TACAGCACAA GCAACTGGAG CAGAAGCTGA AGCAAGCAGA GGCCTGGTGG CTGCAGCTGG 1621 GAAAGTTTGC CCGCCTGGTT GACTACATGA TTTGTCAGAG CCTCATTTCT GTCTTGGAAG 1681 AGCAGATAAC CTCTTTTGTG GCCAACATCC TTCAAGCCCC AAGGCAGAAA CCCTTTCTCT 1741 CATCACAGCT GGTCTTTGAT GATCATGGTC AACTGTCTCA TGTGCCCTGT GTTGAAAATA 1801 TGATCCAGAC TCTAACTGGA GGCCTACAGT CTGTCAAGAC CTCTGCCTTG CAGGTATTCT 1861 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1921 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1981 TTTATATATC TTGTGGAAAG GACGACTTCT CACTTCCGGT TCCGTTACCA CGCGTTAAGT 2041 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt