Construct: ORF TRCN0000475457
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013104.1_s317c1
- Derived from:
- ccsbBroadEn_10426
- DNA Barcode:
- AGGGGCTATTGCACACTGTATCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTSZ (1522)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475457
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1522 | CTSZ | cathepsin Z | NM_001336.4 | 99.8% | 100% | 621T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaggcgcggg ccagggtggc ggccgcttct gctgctcgtg ctgctggcgg 121 gcgcggcgca gggcggcctc tacttccgcc ggggacagac ctgctaccgg cctctgcggg 181 gggacgggct ggctccgctg gggcgcagca catacccccg gcctcatgag tacctgtccc 241 cagcggatct gcccaagagc tgggactggc gcaatgtgga tggtgtcaac tatgccagca 301 tcacccggaa ccagcacatc ccccaatact gcggctcctg ctgggcccac gccagcacca 361 gcgctatggc ggatcggatc aacatcaaga ggaagggagc gtggccctcc accctcctgt 421 ccgtgcagaa cgtcatcgac tgcggtaacg ctggctcctg tgaagggggt aatgacctgt 481 ccgtgtggga ctacgcccac cagcacggca tccctgacga gacctgcaac aactaccagg 541 ccaaggacca ggagtgtgac aagtttaacc aatgtgggac atgcaatgaa ttcaaagagt 601 gccacgccat ccggaactac accctctgga gggtgggaga ctacggctcc ctctctggga 661 gggagaagat gatggcagaa atctacgcaa atggtcccat cagctgtgga ataatggcaa 721 cagaaagact ggctaactac accggaggca tctatgccga ataccaggac accacatata 781 taaaccatgt cgtttctgtg gctgggtggg gcatcagtga tgggactgag tacTGGATTG 841 TCCGGAATTC ATGGGGTGAA CCATGGGGCG AGAGAGGCTG GCTGAGGATC GTGACCAGCA 901 CCTATAAGGA TGGGAAGGGC GCCAGATACA ACCTTGCCAT CGAGGAGCAC TGTACATTTG 961 GGGACCCCAT CGTTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGGGGCTATT GCACACTGTA 1141 TCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt