Construct: ORF TRCN0000475507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017506.1_s317c1
- Derived from:
- ccsbBroadEn_13840
- DNA Barcode:
- GTCGAAACTTACACGTGGCACTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2F1 (1572)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_011526555.3 | 75.1% | 73.7% | (many diffs) |
| 2 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_011526553.2 | 68.4% | 66.4% | (many diffs) |
| 3 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | NM_000774.5 | 65.1% | 61.2% | 843C>G;906_963del;1019_1473del |
| 4 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_011526551.3 | 64.1% | 60.4% | 843C>G;906_963del;1019_1494del |
| 5 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_011526552.3 | 64.1% | 60.4% | 843C>G;906_963del;1019_1494del |
| 6 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_017026386.1 | 60.4% | 43.3% | (many diffs) |
| 7 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_017026387.1 | 60.4% | 43.3% | (many diffs) |
| 8 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XR_935755.1 | 58.6% | 1_215del;1058C>G;1176_1636del | |
| 9 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_017026385.1 | 56.4% | 53.2% | (many diffs) |
| 10 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_017026384.2 | 55.7% | 52.5% | (many diffs) |
| 11 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | NR_135528.2 | 53.6% | 1_72del;915C>G;1033_1789del | |
| 12 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XR_430190.3 | 52.5% | 1_403del;1246C>G;1364_1824del | |
| 13 | human | 1572 | CYP2F1 | cytochrome P450 family 2 su... | XM_011526554.3 | 51.3% | 47.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacagcata agcacagcca tcttactcct gctcctggct ctcgtctgtc 121 tgctcctgac cctaagctca agagataagg gaaagctgcc tccgggaccc agacccctct 181 caatcctggg aaacctgctg ctgctttgct cccaagacat gctgacttct ctcactaagc 241 tgagcaagga gtatggctcc atgtacacag tgcacctggg acccaggcgg gtggtggtcc 301 tcagcgggta ccaagctgtg aaggaggccc tggtggacca gggagaggag tttagtggcc 361 gcggtgacta ccctgccttt ttcaacttta ccaagggcaa tggcatcgcc ttctccagtg 421 gggatcgatg gaaggtcctg agacagttct ctatccagat tctacggaat ttcgggatgg 481 ggaagagaag cattgaggag cgaatcctag aggagggcag cttcctgctG GCGGAGCTGC 541 GGAAAACTGA AGGCGAGCCC TTTGACCCCA CGTTTGTGCT GAGTCGCTCA GTGTCCAACA 601 TTATCTGTTC CGTGCTCTTC GGCAGCCGCT TCGACTATGA TGATGAGCGT CTGCTCACCA 661 TTATCCGCCT TATCAATGAC AACTTCCAAA TCATGAGCAG CCCCTGGGGC GAGTTGTACG 721 ACATCTTCCC GAGCCTCCTG GACTGGGTGC CTGGGCCGCA CCAACGCATC TTCCAGAACT 781 TCAAGTGCCT GAGAGACCTC ATCGCCCACA GCGTCCACGA CCACCAGGCC TCGCTAGACC 841 CCAGATCTCC CCGGGACTTC ATCCAGTGCT TCCTCACCAA GATGGCAGAG GAGAAGGAGG 901 ACCCACTGAG GCACTTCCAC ATGGATACCC TGCTGATGAC CACACATAAC CTGCTCTTTG 961 GCGGCACCAA GACGCCCGCG TGCAGGAGGA GATCGACCTC GTGGTGGGAC GCGCGCGGCT 1021 GCCGGCGCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTCGAA ACTTACACGT GGCACTCCAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt