Construct: ORF TRCN0000475615
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014491.1_s317c1
- Derived from:
- ccsbBroadEn_10160
- DNA Barcode:
- CCTGGAGCGGTCCGACTCCCTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHG7 (440107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475615
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440107 | PLEKHG7 | pleckstrin homology and Rho... | NM_001004330.2 | 99.9% | 100% | 708G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1206
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatctttatg aatacactaa gatatctgca aactcatgaa tatctcctag 121 atgtggattt atggagactt tttgcaaacc tggaggagtt aactcagaca agccttggtt 181 ttgtgaacag tctctttggc atcatcaagg actatgtaga cgcttctgag atttcctcat 241 cactggattt tatttccgtg ctcacaaagt atttccgagg gagtctctgt cagagccacc 301 agacctactg cctgaactat tcagctgcta tcttttatct tgagagcctg aggcagagag 361 atgactttgg aatttattta aaatggtgtg agcagaatga acaatgcaga cggctccacg 421 tgccagagct gctagtggcc ccactacaga ggctcactcg atatccgttg ttgctgaaga 481 atatctggaa aaggagcatg gactctgctg agaaaatcat gatctactcc atcaaggaaa 541 aggtggaaaa gtccatccgg gaccttgaag gaaaagtgaa gtggctggac aatttccaaa 601 aatttagata tctacaggag attatagtgt ggccaccgct ttgggataga gataaaaggt 661 ttttcattcc agagtgtttg aaacacattt ttaaagaaca catggcagaa aacatcttgt 721 caccaaccag cagacacctt ctctatgaag gaaaattaac tcttgcagaa agcacaagat 781 tcctagatgt ttatctgttt ctcttcaatg atttcctctt agttacgaaa actaagtgca 841 acaaaaagaa acttggaggc tcagaccctg gtttaatgtg tccttctctt actccTGAGT 901 TGCAAGCAGT AATAAAAGAG GGTGGTTCGT GTACAGTACT CGATCAGCCT ATTCCACTAG 961 ATAGATTGGT AGTCAAAAGT ATTGAACCAC TCCATGTGTC AGTCTTTGGG CTGAGAAATG 1021 CTTTTCTTAT ACAACACGAA AACAGATATC GACAGTGTAT AGCAGCATTC TTATTACAAG 1081 CCCAAACGGA AAACATCAAA AAAACATGGA TGGCACAAAT AACAACTGCA ATTTCTTGCT 1141 TTACCAAGAG TCAGGAAACC AAGAAAATAT CTTTATTCAC ATTGCCCGCA GAATCCTCTG 1201 AAATTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTGGAGCG GTCCGACTCC CTACTACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt