Construct: ORF TRCN0000475645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005924.1_s317c1
- Derived from:
- ccsbBroadEn_10564
- DNA Barcode:
- ACCAGGGGCCCTCTTTAGGCATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC151760 (151760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151760 | LOC151760 | putative uncharacterized pr... | XR_001740844.1 | 46.2% | 1_582del;1121C>T;1180_1288del | |
| 2 | human | 151760 | LOC151760 | putative uncharacterized pr... | XR_924329.2 | 9.8% | 1_5315del;5854C>T;5913_6021del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 666
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttccagtc cagaggagaa agctagaccc actcctcaaa aagtataggc 121 atcataagaa ggccacaagg acaaaaagaa ggaggaagga gaaaatggag gcccagtgct 181 cccctgttcc tccaacacct tcaacaccac cacaaagtga agaagatgag gctgtagaca 241 aaaagccaac tctactcagt gcccaggaag atactcctga ccttctccat gaggacagat 301 tacagtatct gcaggaagag ggttccggtg tgatgcatca ggaatgtcag atccagtcct 361 gtgagctctc ggtggctcag aagcccaggc cctcttctcc tgcagtgaca tccttggcat 421 caccaccact ctgctttggc agtttcctaa gctgtgtctg ccagaccttc tcaaggtcta 481 ggaagcagaa gcctcccaga agaaagggta acaaccaggc TGAGGCAGGA GGTGATGCTG 541 AGGTCCTGAG ACCTGGCCCG GCAAAACCAG AGTTGAGCCT CAGCAAAACG TGTAGCCACT 601 TGAAATTGAT AGAGCTTTCC TTTGTGTTTA GTTTTATAGT TCTCTCTGTA TGTCATTGTT 661 CCTCTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 721 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 781 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCAGGGGC CCTCTTTAGG CATCGACGCG 841 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt