Construct: ORF TRCN0000475658

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002232.1_s317c1
Derived from:
ccsbBroadEn_10658
DNA Barcode:
TGGTACTTATCGTGGTGGAGTCTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRT16P3 (644945)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475658
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3868 KRT16 keratin 16 NM_005557.4 17.5% 17.1% (many diffs)
2 human 400578 KRT16P2 keratin 16 pseudogene 2 NR_029392.1 16% (many diffs)
3 human 3861 KRT14 keratin 14 NM_000526.5 16% 15.4% (many diffs)
4 human 729252 KRT16P1 keratin 16 pseudogene 1 NR_073414.1 13.9% (many diffs)
5 human 644945 KRT16P3 keratin 16 pseudogene 3 NR_029393.1 12.4% 1_437del;693_2051del
6 mouse 16664 Krt14 keratin 14 NM_001313956.1 17.1% 16.5% (many diffs)
7 mouse 16666 Krt16 keratin 16 NM_008470.1 16.1% 15.5% (many diffs)
8 mouse 16666 Krt16 keratin 16 NM_001313958.1 16% 15.5% (many diffs)
9 mouse 16664 Krt14 keratin 14 NM_016958.2 14.9% 14.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
324
ORF length:
255
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcagcacctc ggtgaccgcc tggcctccta cctggacaag gtgcgtgctc
121tggaggaggc caacgccgac ctgGAAGTGA AGATCCGTGA CTGGTACCAG AGGCAGCGGC
181CCAGTGAGAT CAAAGACTAC AGTCCCTACT TCAAGACCAT CGAGGACCTG AGGCACAAGA
241TCATTGCGGC CACCATTGAG AATGCGCAGC CCATTTTGCA GATTGACAAT GCCAGGCTGG
301CAGCTGATGA CTTCAGGACC AATTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
361AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
421TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GGTACTTATC
481GTGGTGGAGT CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
541att

Download FASTA (ORF) (Full)