Construct: ORF TRCN0000475658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002232.1_s317c1
- Derived from:
- ccsbBroadEn_10658
- DNA Barcode:
- TGGTACTTATCGTGGTGGAGTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT16P3 (644945)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475658
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3868 | KRT16 | keratin 16 | NM_005557.4 | 17.5% | 17.1% | (many diffs) |
| 2 | human | 400578 | KRT16P2 | keratin 16 pseudogene 2 | NR_029392.1 | 16% | (many diffs) | |
| 3 | human | 3861 | KRT14 | keratin 14 | NM_000526.5 | 16% | 15.4% | (many diffs) |
| 4 | human | 729252 | KRT16P1 | keratin 16 pseudogene 1 | NR_073414.1 | 13.9% | (many diffs) | |
| 5 | human | 644945 | KRT16P3 | keratin 16 pseudogene 3 | NR_029393.1 | 12.4% | 1_437del;693_2051del | |
| 6 | mouse | 16664 | Krt14 | keratin 14 | NM_001313956.1 | 17.1% | 16.5% | (many diffs) |
| 7 | mouse | 16666 | Krt16 | keratin 16 | NM_008470.1 | 16.1% | 15.5% | (many diffs) |
| 8 | mouse | 16666 | Krt16 | keratin 16 | NM_001313958.1 | 16% | 15.5% | (many diffs) |
| 9 | mouse | 16664 | Krt14 | keratin 14 | NM_016958.2 | 14.9% | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 324
- ORF length:
- 255
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagcacctc ggtgaccgcc tggcctccta cctggacaag gtgcgtgctc 121 tggaggaggc caacgccgac ctgGAAGTGA AGATCCGTGA CTGGTACCAG AGGCAGCGGC 181 CCAGTGAGAT CAAAGACTAC AGTCCCTACT TCAAGACCAT CGAGGACCTG AGGCACAAGA 241 TCATTGCGGC CACCATTGAG AATGCGCAGC CCATTTTGCA GATTGACAAT GCCAGGCTGG 301 CAGCTGATGA CTTCAGGACC AATTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 361 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 421 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GGTACTTATC 481 GTGGTGGAGT CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 541 att