Construct: ORF TRCN0000475705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004109.1_s317c1
- Derived from:
- ccsbBroadEn_06267
- DNA Barcode:
- TGCCATGTATATCCAGGCCACCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCNT2 (2651)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2651 | GCNT2 | glucosaminyl (N-acetyl) tra... | NM_145655.3 | 99.8% | 99.7% | 816C>G;834A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1275
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacttttgg aggtactgct tttttgcttt cactctgctc agcgtggtca 121 tttttgtgag attttacagt agccaattga gcccgccaaa aagttatgag aagctgaaca 181 gttccagtga aaggtatttt aggaaaactg cctgtaatca cgccttagag aaaatgccag 241 tctttttgtg ggaaaatata ttaccatcac ctttgcgaag tgtcccttgc aaggattacc 301 tgacccagaa tcactacatc acaagtcccc tgtcggaaga agaggctgca ttccctttgg 361 cctatgtcat ggtcatccat aaggactttg acacctttga aaggctcttt agggctatct 421 atatgcccca aaatgtctac tgtgttcacg tggatgagaa agccccagct gagtataagg 481 aatctgtgag gcagttactg agttgcttcc aaaatgcttt cattgcttca aagacagagt 541 ctgtggttta tgcaggcatt tccagactcc aggctgacct gaactgtctg aaagaccttg 601 tcgcctctga ggttccctgg aagtacgtca tcaacacctg tggacaagac ttccccctga 661 aaaccaaccg ggagatagtt cagcatctga aaggatttaa agggaaaaat atcaccccag 721 gggtgctgcc tcctgaccat gcaattaagc gaactaaata tgtccaccaa gagcatacag 781 ataaaggtgg cttttttgtg aaaaatacta atattttgaa aacttcacct ccacatcagc 841 tgaccatcta ctttggcact gcctatgtgg cgcttaccag agagtttgtc gactttgttc 901 tgcgtgacca aagggccatt gatctactac aatggtcaaa agatacctat agtccTGATG 961 AGCATTTCTG GGTGACACTT AATAGGGTTT CAGGTGTTCC TGGCTCTATG CCAAATGCAT 1021 CCTGGACTGG AAACCTCAGA GCTATAAAGT GGAGTGACAT GGAAGACAGA CACGGAGGCT 1081 GCCACGGCCA CTATGTACAT GGTATTTGTA TCTATGGAAA CGGAGACTTA AAGTGGCTGG 1141 TTAATTCACC AAGCCTGTTT GCTAACAAGT TTGAGCTTAA TACCTACCCC CTTACTGTGG 1201 AATGCCTAGA ACTGAGGCAT CGCGAAAGAA CCCTCAATCA GAGTGAAACT GCGATACAAC 1261 CCAGCTGGTA TTTTTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCCATGTAT ATCCAGGCCA 1441 CCGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt