Construct: ORF TRCN0000475717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000240.1_s317c1
- Derived from:
- ccsbBroadEn_11884
- DNA Barcode:
- TGACTGCATTATGTCCGAGCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGLV3-21 (28796)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001178126.2 | 63.6% | 53.4% | (many diffs) |
| 2 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 54.5% | 51% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 768
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctggacc gttctcctcc tcggcctcct ctctcactgc acagtctctg 121 tgacttccta tgtgctgact cagccaccct cggtgtcagt ggccccagga cagacggcca 181 ggattacttg tgggggaaac aacattggaa gtaaaagtgt gcactggtac cagcagaagc 241 caggccaggc ccctgtcatg gtcgtctatg atgatagtga ccggccctca ggaatccctg 301 agcgactctc tggctccaac tctgggaaca cggccaccct gaccatcagc agggtcgaag 361 ccggggatga ggccgactat tactgtcagg tctgggacag tactagtaat cattatgtct 421 tcggaactgg gaccaaggtc tccgtcctaa gtcagcccaa ggccaacccc actgtcactc 481 tgttcccgcc cTCCTCTGAG GAGCTCCAAG CCAACAAGGC CACACTAGTG TGTCTGATCA 541 GTGACTTCTA CCCGGGAGCT GTGACAGTGG CCTGGAAGGC AGATGGCAGC CCCGTCAAGG 601 CGGGAGTGGA GACCACCAAA CCCTCCAAAC AGAGCAACAA CAAGTACGCG GCCAGCAGCT 661 ACCTGAGCCT GACGCCCGAG CAGTGGAAGT CCCACAGAAG CTACAGCTGC CAGGTCACGC 721 ATGAAGGGAG CACCGTGGAG AAGACAGTGG CCCCTACAGA ATGTTCATTG CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGATGACTGC ATTATGTCCG AGCTATCACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt