Construct: ORF TRCN0000475786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005739.1_s317c1
- Derived from:
- ccsbBroadEn_09859
- DNA Barcode:
- TTCTCCTACCTCTCAGATGATCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DLEU7 (220107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220107 | DLEU7 | deleted in lymphocytic leuk... | NM_198989.3 | 99.7% | 99.3% | 248C>T |
| 2 | human | 220107 | DLEU7 | deleted in lymphocytic leuk... | NM_001306135.1 | 71.3% | 69.6% | (many diffs) |
| 3 | human | 220107 | DLEU7 | deleted in lymphocytic leuk... | XR_001749491.2 | 10.9% | (many diffs) | |
| 4 | mouse | 239133 | Dleu7 | deleted in lymphocytic leuk... | NM_173419.2 | 57.8% | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 549
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccagccct gcgcccttag tggcctccat cagccaccaa atggtggctc 121 tgcagacctt gcagctgctg cagcaggagt ggggctgggg ggacggtcca gtcgcccccg 181 ggaacccgcg ggacccagac cacgtgtcca ccgctccagc ccgtcgctca ggccCGCCGC 241 GGGCCCGGCC GGGGCCCGGG CGCGAGGAGC GGGGCGGGGG CGTGGGGACC AGGAGTCGGC 301 GGACCGCGGC GCGGGTGAAC TCCCCAGAGG AGGAGGTAGT GCGAGGCGCT GAGGGGGGCG 361 CCGAGTTGCT GCCCTTCCCC CGGGACCGCG GGCCCTGCAC CCTGGCCCAG ATGGCGATGC 421 GCAGCGCGCT GGCCCGCGTG GTGGACTCGA CTTCGGAGCT GGTCAGCGTG GAGCAGACGC 481 TGCTGGGGCC CCTCCAGCAG GAGCGGTCCT TTCCCATTCA CCTGAAGCTT CAACTCCTGG 541 CTAATGAGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 601 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 661 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCTCC TACCTCTCAG ATGATCGGAC 721 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt