Construct: ORF TRCN0000475817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006662.1_s317c1
- Derived from:
- ccsbBroadEn_09067
- DNA Barcode:
- CGTCAAAACCAGCTTTCTACAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMX1 (81542)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475817
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81542 | TMX1 | thioredoxin related transme... | NM_030755.5 | 99.7% | 100% | 492T>G;648G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 909
- ORF length:
- 840
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgccctcc gggagtcttg cagttcccct ggcagtcctg gtgctgttgc 121 tttggggtgc tccctggacg cacgggcggc ggagcaacgt tcgcgtcatc acggacgaga 181 actggagaga actgctggaa ggagactgga tgatagaatt ttatgccccg tggtgccctg 241 cttgtcaaaa tcttcaaccg gaatgggaaa gttttgctga atggggagaa gatcttgagg 301 ttaatattgc gaaagtagat gtcacagagc agccaggact gagtggacgg tttatcataa 361 ctgctcttcc tactatttat cattgtaaag atggtgaatt taggcgctat cagggtccaa 421 ggactaagaa ggacttcata aactttataa gtgataaaga gtggaagagt attgagcccg 481 tttcatcatg gtttggtcca ggttctgttc tgatgagtag tatgtcagca ctctttcagc 541 tATCTATGTG GATCAGGACG TGCCATAACT ACTTTATTGA AGACCTTGGA TTGCCAGTGT 601 GGGGATCATA TACTGTTTTT GCTTTAGCAA CTCTGTTTTC CGGACTGTTA TTAGGACTCT 661 GTATGATATT TGTGGCAGAT TGCCTTTGTC CTTCAAAAAG GCGCAGACCA CAGCCATACC 721 CATACCCTTC AAAAAAATTA TTATCAGAAT CTGCACAACC TTTGAAAAAA GTGGAGGAGG 781 AACAAGAGGC GGATGAAGAA GATGTTTCAG AAGAAGAAGC TGAAAGTAAA GAAGGAACAA 841 ACAAAGACTT TCCACAGAAT GCCATAAGAC AACGCTCTCT GGGTCCATCA TTGGCCACAG 901 ATAAATCCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 961 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1021 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTCAA AACCAGCTTT CTACAGTCAC 1081 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt