Construct: ORF TRCN0000475885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010071.1_s317c1
- Derived from:
- ccsbBroadEn_07286
- DNA Barcode:
- TAAAAGCATCAAGATTACCCGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT38 (8687)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475885
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8687 | KRT38 | keratin 38 | NM_006771.3 | 99.9% | 99.7% | 1267T>C |
| 2 | human | 8688 | KRT37 | keratin 37 | NM_003770.5 | 91.7% | 90.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1437
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacctcttcc tacagcagct cctcatgccc tctgggttgc accatggctc 121 ctggagcaag aaatgtctct gtctctccca tcgacattgg gtgccagcct ggggcagagg 181 ccaacattgc ccccatgtgc cttttggcca acgtggcaca tgccaaccga gtccgtgtgg 241 ggtccactcc cctgggccgc cccagcctct gtctgccgcc tacctgccac actgcttgtc 301 ccttgccagg gacctgccac attcctggca acattggaat ctgtggggcc tatggtgaaa 361 acaccctgaa tggccatgag aaggagacca tgcagttcct gaatgaccgc ctggccaact 421 acctggagaa ggtgcgccag ctggagcagg agaatgcgga gctggaggcc acactcctcg 481 agaggagcaa gtgccacgag tccaccgtgt gccccgacta ccagtcttac ttccacacca 541 tcgaggagct ccaacagaag atcctgtgca gcaaggccga gaatgccagg ctgattgtac 601 aaattgacaa tgccaagctg gctgccgatg actttaggat caagctggag agtgagcgct 661 ccctgcgcca gctggtggag gcagacaagt gtgggacaca gaagctcctg gatgatgcga 721 ccctggccaa ggccgacctg gaggcccagc aggagtccct gaaggaggag cagctctccc 781 tcaagagcaa ccacgagcag gaagtaaaga ttctgaggag tcagctgggg gagaagctcc 841 ggattgagct ggacattgag cccaccattg acctgaacag ggtgctgggg gagatgcggg 901 ctcagtatga ggccatgttg gagaccaacc gccaggatgt ggaacagtgg ttccaagccc 961 agtctgaagg catcagcctg caggacatgt cctgctccga ggagctgcag tgctgccagt 1021 cggagatcct ggagctgaga tgcacggtga atgccctGGA GGTGGAGCGC CAAGCCCAGC 1081 ACACCTTGAA GGACTGTCTG CAGAACTCCC TGTGTGAAGC CGAGGACCGC TTCGGCACGG 1141 AGCTGGCCCA GATGCAGAGC CTCATCAGCA ACGTGGAGGA GCAGCTGTCT GAGATCCGGG 1201 CCGACCTGGA GCGGCAAAAC CAGGAGTACC AGGTGCTGCT GGACGTGAAG ACCCGGCTGG 1261 AGAATGAGAT TGCCACGTAC CGGAACCTTC TGGAAAGCGA GGACTGCAAA CTCCCCTGCA 1321 ATCCGTGCTC CACGCCTCCC TCCTGCGTGA CTGCCCCCTG TGCTCCTCGC CCAAGCTGTG 1381 GCCCCTGCAC CACCTGTGGG CCCACCTGTG GAGCCAGCAC CACCGGAAGC CGATTCTTGC 1441 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1501 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1561 ATATATCTTG TGGAAAGGAC GATAAAAGCA TCAAGATTAC CCGACCACGC GTTAAGTCga 1621 caatcaacct ctggattaca aaatttgtga aagatt