Construct: ORF TRCN0000475896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006699.1_s317c1
- Derived from:
- ccsbBroadEn_08585
- DNA Barcode:
- GTGGTGCAAATAACGCCCAACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIOK2 (55781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475896
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55781 | RIOK2 | RIO kinase 2 | NM_018343.3 | 99.8% | 99.6% | 430C>T;1636G>C |
| 2 | human | 55781 | RIOK2 | RIO kinase 2 | NM_001159749.2 | 85.3% | 84.6% | (many diffs) |
| 3 | human | 55781 | RIOK2 | RIO kinase 2 | XM_017009628.1 | 66% | 65.9% | 0_1ins561;1075G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaaagtgaat gtggccaagt tgcgttacat gagccgagat gacttcaggg 121 tcttgaccgc ggttgaaatg ggcatgaaga accatgaaat tgttcccggc agtttgattg 181 cttctatagc cagccttaaa catggtggct gtaataaagt tttaagagaa ttagtgaaac 241 ataaactcat agcttgggag cgtaccaaaa ctgtccaggg ctatcggttg acaaatgcag 301 gatatgatta cctagctttg aaaacacttt cttctaggca agtagttgag tctgttggaa 361 accagatggg tgttggcaaa gaatcagata tttacattgt tgcaaatgaa gaaggacaac 421 aatttgcatt aaagcttcac agactaggaa gaacctcgtt tcgaaatttg aaaaacaaac 481 gcgattatca taaatatagg cacaatgtgt catggctata tttatctcgt ctctctgcca 541 tgaaggaatt tgcctatatg aaggcattgt atgagaggaa atttccagtt ccaaagccaa 601 ttgattacaa tcgtcatgca gtggtcatgg aactcataaa tggttatcca ctatgtcaga 661 tacaccatgt tgaagatcct gcatcagtat atgatgaagc tatggaacta attgtcaaac 721 ttgcaaatca tgggctgatt catggagatt ttaatgaatt taatctcatt ttggatgaaa 781 gtgaccatat caccatgatt gattttccac agatggtttc aacttctcat cccaatgctg 841 agtggtattt tgacagagat gttaaatgca ttaaagattt ctttatgaaa cgtttcagct 901 acgaaagtga gctttttcca acttttaagg atatcaggag agaagacact cttgatgtgg 961 aggtttctgc cagtggctac acaaaggaaa tgcaggcaga tgatgaactg cttcatccat 1021 taggtccaga tgataaaaat attgaaacaa aagagggatc tgaattctca ttttcagatg 1081 gagaagtggc agaaaaagca gaggtttacg ggtcagaaaa tgaaagtgaa cggaactgtc 1141 tagaagaatc agagggctgc tattgcagat catctggaga ccctgaacaa ataaaggaag 1201 acagtttatc agaagagagt gctgatgcac ggagttttga aatgactgaa ttcaatcaag 1261 ctttagaaga aataaaaggg caggttgttg aaaacaactc tgtaactgaa ttttctgagg 1321 agaaaaacag aactgaaaat tacaacaggc aagatggtca gagagttcaa ggaggagtcc 1381 ctgctggctc tgacgagtat gaagatgaat gccctcatct aattgccttG TCGTCATTAA 1441 ATAGAGAATT CAGGCCTTTC AGAGATGAAG AAAATGTGGG AGCTATGAAT CAGTATAGAA 1501 CAAGAACTCT GAGTATCACT TCTTCAGGCA GTGCTGTAAG CTGTTCAACA ATTCCTCCAG 1561 AACTGGTGAA ACAGAAGGTG AAACGTCAGT TGACAAAACA GCAAAAATCA GCTGTCAGAC 1621 GTCGATTGCA GAAAGGAGAA GCAAATATAT TTACCAAGCA ACGTAGGGAA AACATGCAAA 1681 ATATCAAATC AAGTTTGGAA CCAGCCAGCT TTTGGGGAGA ATACCCAACT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGAGTG GTGCAAATAA CGCCCAACTG TACGCGTTAA GTCgacaatc aacctctgga 1921 ttacaaaatt tgtgaaagat t