Construct: ORF TRCN0000475898
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010847.1_s317c1
- Derived from:
- ccsbBroadEn_13873
- DNA Barcode:
- CCATAGCTGGACGCGAGAGTTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXB1 (3211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475898
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3211 | HOXB1 | homeobox B1 | NM_002144.4 | 71.4% | 66% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 765
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggactataat tggatgaact ccttcttaga gtacccactc tgtaaccggg 121 gacccagcgc ctacagcgcc cacagcgccc caacctcctt tcccccaagc tcggctcagg 181 cggttgacag ctatgcaagc gagggccgct acggtggggg gctgtccagc cctgcgtttc 241 agcagaactc cggctatccc gcccagcagc cgccttcgac cctgggggtg cccttcccca 301 gctccgcgcc ctcggggtat gctcctgccg cctgcagccc cagctacggg ccttctcagt 361 actaccctct gggtcaatca gaaggagacg gaggctattt tcatccctcg agctacgggg 421 cccagctagg gggcttgtcc gatggctacg gagcaggtgg agccggtccg gggccatatc 481 ctccgcagca tcccccttat gggaacgagc agaccgcgag ctttgcaccg gcctatgctg 541 atctcctctc cgaggacaag gaaacaccct gcccttcaga acctaacacc cccacggccc 601 ggaccttcga ctggatgaag gttaagagaa acccacccaa gacaggtagg gctcagatgt 661 ggccacccct tctccggggc ccaaagcatc tctgcttccc ctgcagtgac atgtcctggg 721 tcTGGGCTGG GTTCTTCTCT TTCAGCGGCT CTGGTAGACA CAGGTTGCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA CCATAGCTGG ACGCGAGAGT TATGACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt