Construct: ORF TRCN0000475941
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010535.1_s317c1
- Derived from:
- ccsbBroadEn_09970
- DNA Barcode:
- CGTTATGGCCCCGCGAAGCATCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTBD8 (284697)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475941
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284697 | BTBD8 | BTB domain containing 8 | NM_183242.4 | 99.8% | 99.4% | 610T>G;1127G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1203
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcgctgt ggggaaggca gtgcggcccc catggtactt ctggggtccg 121 ctggagtttg cagtaagggg ttgcaaagga aggggccgtg tgagcggcgc cggctgaagg 181 cgacggtgtc ggagcagctc agccaggatt tgctcaggct tctaagggaa gaattccata 241 cagatgttac cttctctgtg ggttgtactt tgttcaaagc acacaaagca gtccttttag 301 caagagttcc tgacttctat tttcatacta ttggacagac atcaaatagt ttaacaaatc 361 aggagcctat tgctgtggag aatgttgaag ctttagaatt tagaacgttt ttacagatta 421 tatattcatc aaacagaaac ataaaaaact atgaagagga aattcttagg aaaaagataa 481 tggagattgg gatatcacaa aagcaacttg acatcagttt tccaaagtgt gaaaactcat 541 ctgattgttc tcttcagaag catgaaattc cagaggatat cagtgacaga gatgatgatt 601 tcatttccaa tgataattat gacttggagc ctgcatctga attaggagaa gatttattga 661 agctttatgt gaaacctggt tgcccagata ttgatatttt tgttgatgga aaacgtttta 721 aagctcacag ggccattttg agtgccagat ctagttattt tgctgcaatg ctgagtggct 781 gttgggctga aagctcccaa gagtacgtta ctcttcaagg tataagccat gtagaactga 841 atgttatgat gcattTTATA TATGGAGGAA CTCTGGACAT TCCAGACAAA ACTAATGTTG 901 GTCAGATACT CAATATGGCT GATATGTATG GACTAGAAGG ATTAAAAGAA GTAGCAATCT 961 ATATTTTAAG AAGAGATTAC TGTAATTTCT TTCAGAAGCC TGTTCCCAGA ACATTGACGT 1021 CTATACTAGA ATGCCTGATT ATTGCTCATT CAGTTGGAGT GGAAAGTCTT TTTGCTGACT 1081 GCATGAAGTG GATTGTAAAG CATTTTGCAA GGTTTTGGTC TGAGAGAAGC TTTGCAAATA 1141 TACCTCCTGA GATTCAGAAA AGTTGTCTTA ATATGTTGAT TCAGTCCTTA GTAAATATAA 1201 CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGACG TTATGGCCCC GCGAAGCATC TGACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt