Construct: ORF TRCN0000475980
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003901.1_s317c1
- Derived from:
- ccsbBroadEn_02707
- DNA Barcode:
- TTTGTATGCTCCGAAGTGTGTTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC4A1AP (22950)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475980
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22950 | SLC4A1AP | solute carrier family 4 mem... | NM_018158.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2457
- ORF length:
- 2388
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttagcacca cttcgcaacg ctccaggtcg tgaaggagca acttcaccat 121 cgccgcctac agacgccact gggagcttgg gagagtggga cgtggacagg aacgtaaaga 181 ccgaagggtg ggtttcgaag gagcggattt cgaagttgca ccggttgagg atggctgaca 241 ttctctctca gtcagagacc ctggcgtcgc aagacctcag tggggacttc aagaagccag 301 ctctgccggt gtccccagcg gcgcggagta aggccccggc cagcagttct tcaaaccctg 361 aggaggtaca gaaggaaggg cccactgcgt tgcaggactc caattctggg gagcccgaca 421 tccctcctcc tcagccggac tgcggtgatt ttaggagtct acaggaggag cagtcgcgcc 481 ccccgacagc ggtttcttcc cctggcggtc cagcccgggc tcccccctac caagagcctc 541 catggggtgg ccctgccaca gccccctaca gcttagagac cctgaagggc ggcactatcc 601 ttggcacccg tagcttgaaa gggacgagtt actgcctttt cgggaggctg tctggctgcg 661 acgtgtgcct ggagcaccct tcggtgtctc ggtaccacgc agtgctgcag cacagggcgt 721 ccggccctga cggagaatgc gacagcaacg ggccgggctt ctacctctac gatctgggaa 781 gcacccatgg cacttttctc aacaaaactc gcatcccacc tcgcacctac tgtcgagtcc 841 acgttgggca tgttgttcgc tttggaggca gcacccggct ctttatcctg cagggaccag 901 aggaagaccg agaggcagaa tccgagttaa cagtaacaca gttgaaggaa ttgcgcaagc 961 agcagcaaat attgttggag aagaagatgc taggagaaga ctcagatgaa gaagaggaaa 1021 tggatacctc tgaaaggaag ataaatgctg gtagccaaga tgatgagatg ggttgcacct 1081 ggggaatggg agaagatgca gtagaggatg atgctgaaga gaaccctatt gtcttagagt 1141 ttcagcagga aagggaggcc ttttatataa aggatcccaa aaaggctctc caaggctttt 1201 ttgaccgaga aggagaagaa ttagaatatg aatttgatga acagggacat agcacttggc 1261 tctgcagggt gagattacct gtggacgatt caactggaaa acaactggtg gctgaggcca 1321 ttcactcagg aaagaaaaaa gaagcaatga tccagtgctc attggaagct tgtcggattc 1381 ttgacacttt gggattgctt cggcaggaag cagtatctcg gaaaaggaaa gccaagaact 1441 gggaagatga agacttttat gatagtgatg atgacacatt tcttgatagg actggcctga 1501 ttgagaagaa gcgtctgaac agaatgaaga aggctggcaa gattgatgag aagccagaga 1561 cctttgaatc attggttgca aaattaaatg atgctgaaag ggaactttct gaaatttctg 1621 agagattgaa agcctcaagc caagttctat cagagtctcc atctcaggat tctttagatg 1681 cgttcatgtc agaaatgaaa tcaggcagta cattagatgg tgtgtcccgg aagaaacttc 1741 acctgagaac ttttgaactg aggaaagaac aacagagact taaagggtta ataaaaattg 1801 taaagccagc agagattcca gaactaaaaa agactgaaac tcagactaca ggtgcagaaa 1861 acaaagctaa aaagcttaca ttgcctctat ttggtgccat gaaaggagga agcaaattca 1921 aattaaaaac tggaacagta gggaagttac cccccaagcg tccagaactc cctccaactc 1981 taatgagaat gaaagatgag cctgaagtag aagaggagga ggaagaggaa gaggaagaag 2041 agaaagaaaa ggaggagcat gaaaagaaaa aactggagga tggaagccTC AGTAGGCCAC 2101 AGCCAGAGAT AGAGCCAGAA GCAGCAGTGC AGGAAATGAG GCCTCCCACA GATCTCACAC 2161 ATTTTAAAGA AACCCAAACC CATGAAAACA TGTCTCAACT TAGCGAGGAA GAACAGAATA 2221 AAGATTATCA AGACTGTAGC AAAACCACTT CATTGTGCGC AGGACCCTCA GCATCAAAGA 2281 ATGAATATGA GAAAAGCAGA GGTGAATTGA AGAAAAAGAA AACACCTGGT CCAGGCAAAC 2341 TTCCACCAAC ACTTTCTTCC AAATATCCTG AAGATGACCC AGACTACTGT GTGTGGGTCC 2401 CACCTGAAGG TCAAAGTGGA GATGGCAGAA CCCATCTTAA TGACAAGTAT GGCTATTTGC 2461 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2521 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2581 ATATATCTTG TGGAAAGGAC GATTTGTATG CTCCGAAGTG TGTTGGACGC GTTAAGTCga 2641 caatcaacct ctggattaca aaatttgtga aagatt