Construct: ORF TRCN0000476051
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006212.1_s317c1
- Derived from:
- ccsbBroadEn_10466
- DNA Barcode:
- CGGCGATTCCTGTTACTCATATCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOL9 (79707)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476051
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79707 | NOL9 | nucleolar protein 9 | NM_024654.5 | 99.7% | 99.2% | (many diffs) |
| 2 | human | 79707 | NOL9 | nucleolar protein 9 | XM_005263493.4 | 58.6% | 58.1% | (many diffs) |
| 3 | human | 79707 | NOL9 | nucleolar protein 9 | XM_011542147.3 | 47.1% | 47% | 0_1ins1110;992_993delTG |
| 4 | human | 79707 | NOL9 | nucleolar protein 9 | XM_017002338.2 | 47.1% | 47% | 0_1ins1110;992_993delTG |
| 5 | human | 79707 | NOL9 | nucleolar protein 9 | XM_017002339.2 | 47.1% | 47% | 0_1ins1110;992_993delTG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2175
- ORF length:
- 2106
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggactcg ggactgctgc taaagtgggg ttcctgccgt tccacttggc 121 tgcgggtccg caaggcccgg ccccagctca tcctcagccg ccggccccgc cgccggctcg 181 ggagcctgcg ctggtgcggt cggcggcgcc tacggcggcg gttactgcaa gcccaggcgg 241 ccggcgtgga ctggagggag ggagcccgcc aggtgtcgcg cgcggcggcg gcccggagac 301 ccaacaccgc gacccccagc ccgatcccta gcccgacccc ggcctccgaa cccgagtccg 361 aacccgaact cgaatccgcc tcgagttgcc accggcctct cctcatccca ccggtgcggc 421 ccgtgggccc cggccgcgcg ttgctgctgc tgccggtcga gcagggtttt acttttagtg 481 ggatctgtcg tgtgacttgc ctctatggcc aggtgcaggt atttggtttt accatcagcc 541 aaggccagcc tgcccaagac atcttctctg tgtataccca ctcttgcttg agtatccatg 601 cacttcacta ctcacagcct gagaaaagca agaaggaact gaaaagggaa gcccggaatt 661 tgctcaaatc tcatcttaac cttgatgaca ggcgttggtc gatgcagaat ttttctcctc 721 agtgttccat tgtgttgcta gaacatctga aaactgccac tgtaaacttc ataaccagct 781 atccgggttc atcctacatt tttgtgcaag agagtccaac tccccagatt aaacctgaat 841 atttagcctt gaggtctgtt ggcatcagaa gagagaaaaa aaggaaaggc cttcagttaa 901 ctgagagtac cctttcagcc ctggaagagt tagtcaatgt ttcctgtgaa gaagtagatg 961 gctgccctgt cattctagtt tgtggatccc aggatgttgg aaagtcaaca tttaatagat 1021 acctgattaa ccatttgtta aatagtcttc cctgcgttga ctatttggaa tgtgatctgg 1081 gacagacaga atttacccct cctggttgca tttctttgct taatattaca gaaccagttc 1141 tgggaccacc tttcactcac ctgaggactc cacagaagat ggtatattat gggaaacctt 1201 cttgtaaaaa caactatgag aattatattg acatagtgaa atatgtgttc agcgcttaca 1261 agagagagtc ccctctcatc gtcaacacta tgggatgggt ttcagaccag gggctcctgc 1321 ttctcattga tctgatccga ttgctgtctc ccagccacgt ggttcagttc cgctctgacc 1381 acagtaaata tatgccagac cttaccccgc agtatgtaga tgacatggat ggcttgtaca 1441 caaaaagcaa gaccaagatg agaaatcgac gtttcagact cgcagcattt gcagatgctt 1501 tggaatttgc tgatgaagaa aaagagagtc cagttgagtt cactggacat aaactgatag 1561 gtgtttatac agactttgca ttcagaataa ctccaagaaa tagagagtca cataacaaaa 1621 ttcttcgaga tctgtccatc ttgagttacc ttagccagct gcagcccccg atgcccaaac 1681 cactttctcc tttacatagc ctgacaccct atcaggtccc tttcaatgca gtcgcactcc 1741 ggattaccca ctctgatgtc gcccctaccc atatactata tgctgtaaac gccagctggg 1801 ttggtctttg caagatccag gatgacgtca gaggatacac gaatgggccc atCCTGCTTG 1861 CCCAGACTCC AATCTGTGAC TGCTTGGGCT TTGGCATCTG TAGAGGCATT GACATGGAGA 1921 AGCGGCTGTA CCACATCCTC ACCCCTGTGC CCCCGGAAGA GCTAAGGACC GTGAATTGTC 1981 TGCTCGTTGG AGCTATTGCC ATTCCACATT GTGTCCTTAA GTGCCAGCGT GGGATCGAAG 2041 GGACAGTACC TTATGTCACA ACGGATTACA ATTTTAAACT TCCTGGAGCA TCAGAGAAAA 2101 TTGGAGCAAG AGAACCTGAG GAGGCACATA AAGAGAAACC ATACCGAAGA CCTAAGTTCT 2161 GTCGAAAAAA AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT 2221 AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GCCCGTAACT TGAAAGTATT 2281 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG GCGATTCCTG TTACTCATAT 2341 CTACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt