Construct: ORF TRCN0000476054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005261.1_s317c1
- Derived from:
- ccsbBroadEn_10616
- DNA Barcode:
- GCCTACTGACAAAACAGCTGATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H1FX-AS1 (339942)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476054
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339942 | H1FX-AS1 | H1FX antisense RNA 1 | NR_026991.1 | 13.1% | 1_1373del;1665_2211del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 384
- ORF length:
- 291
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGGATG GGAGCAGGAA ACCCAAAAGA 121 GCCGGCCCTG GAACCAGGTG GAGGGGAGGC AGCCCGGCCA TGACCCAGAG CAGGACACCT 181 GCTCCACTTC ACCTTTTGCC ATGAGTAAAA GCTCCCTGAG GCCTCCCAAG AAGTTGATGC 241 CCTGTGCTTC CTGTACAGCT GCGGAACCGG ATGGCTTTCC GTGGCTTTGC TACAGCCACT 301 CCTGGAAGTG CTGCCTGACT GAATCCTCAG GGCACCCCGG CAGGATGGAC GTGGTGTACC 361 CCCTACTTTA CAGATGGGGA AACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CCTACTGACA 541 AAACAGCTGA TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 601 att