Construct: ORF TRCN0000476138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010876.1_s317c1
- Derived from:
- ccsbBroadEn_10111
- DNA Barcode:
- ATTCACTTTCGTACTCCGATATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SULT6B1 (391365)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476138
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 391365 | SULT6B1 | sulfotransferase family 6B ... | NM_001032377.2 | 99.7% | 99.6% | 336G>C;372T>C |
| 2 | human | 391365 | SULT6B1 | sulfotransferase family 6B ... | NM_001367551.1 | 87.2% | 87.1% | 1_114del;450G>C;486T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 864
- ORF length:
- 795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgcacctca gaaactttcc aagcgctgga caccttcgaa gccagacatg 121 atgacatcgt gctagcatct tatccaaagt gcggttcaaa ctggattctc cacattgtca 181 gtgaattaat atatgctgtt tctaaaaaaa agtataaata tccagaattc ccagttcttg 241 aatgtgggga ttcagaaaaa tatcagagaa tgaaaggctt tccatcacca aggattttgg 301 caactcacct ccactatgac aaattacctg ggtctatctt cgagaataaa gccaagatat 361 tggtgatatt tcgaaaccct aaagatacag cagtatcttt tttccatttc cacaacgatg 421 tccccgatat tccaagctac ggctcttggg atgaattctt cagacagttc atgaaaggac 481 aagtttcttg gggaaggtat tttgattttg caatcaattG GAACAAACAT CTTGATGGCG 541 ACAATGTTAA GTTCATATTA TATGAAGACC TGAAAGAGAA TCTGGCTGCT GGAATAAAAC 601 AGATTGCTGA GTTCTTGGGA TTCTTTCTAA CTGGGGAGCA AATTCAAACT ATCTCAGTCC 661 AGAGCACCTT CCAAGCCATG CGTGCGAAGT CTCAGGACAC ACACGGTGCT GTCGGCCCAT 721 TCCTTTTCCG CAAAGGTGAA GTTGGTGATT GGAAAAATTT GTTCAGTGAA ATTCAGAACC 781 AGGAAATGGA TGAAAAATTC AAAGAGTGCT TAGCAGGCAC CTCCCTCGGA GCAAAGTTGA 841 AGTATGAATC ATATTGCCAG GGTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTCACTTTCG 1021 TACTCCGATA TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att