Construct: ORF TRCN0000476140

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003048.1_s317c1
Derived from:
ccsbBroadEn_00082
DNA Barcode:
GTGCCCTGGATGCACTTCTTGATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
APOC4 (346)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476140
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 346 APOC4 apolipoprotein C4 NM_001646.3 100% 100%
2 human 100533990 APOC4-APOC2 APOC4-APOC2 readthrough (NM... NR_037932.1 20.8% 1_40del;422_1829del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
450
ORF length:
381
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtccctcctc agaaacaggc tccaggccct gcctgcccTG TGCCTCTGCG
121TGCTGGTCCT GGCCTGCATT GGGGCATGCC AGCCAGAGGC CCAGGAAGGA ACCCTGAGCC
181CCCCACCAAA GCTAAAGATG AGTCGCTGGA GCCTGGTGAG GGGCAGGATG AAGGAGCTGC
241TGGAGACAGT GGTGAACAGG ACCAGAGACG GGTGGCAATG GTTCTGGAGC CCGAGCACCT
301TCCGGGGCTT CATGCAGACC TACTATGACG ACCACCTGAG GGACCTGGGT CCGCTCACCA
361AGGCCTGGTT CCTCGAATCC AAAGACAGCC TCTTGAAGAA GACCCACAGC CTGTGCCCCA
421GGCTTGTCTG TGGGGACAAG GACCAGGGTT TGCCAACTTT CTTGTACAAA GTGGTTGATA
481TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
541CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGTGCC
601CTGGATGCAC TTCTTGATCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
661tgaaagatt

Download FASTA (ORF) (Full)

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