Construct: ORF TRCN0000476197
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015687.1_s317c1
- Derived from:
- ccsbBroadEn_04642
- DNA Barcode:
- GGGTACCAGTGCTCAATCACCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAT3 (113179)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476197
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113179 | ADAT3 | adenosine deaminase tRNA sp... | NM_001329533.2 | 100% | 100% | |
| 2 | human | 113179 | ADAT3 | adenosine deaminase tRNA sp... | NM_138422.4 | 95.6% | 95.6% | 1_48del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcccgccccg ggcctcgtgg agcagcccaa gtgcttggag gccgggagcc 121 cggagcctga gccggcgccg tggcaggccc tccctgtcct gtccgagaag cagtcagggg 181 acgtggagct ggtgctggcc tacgccgcgc ccgtcctgga caagcgccag acctcacgcc 241 tcctgaagga ggtgtcggcc ctgcacccgc tccccgccca gcctcacctc aagcgggtgc 301 ggcccagccg cgatgccggc agcccccacg ccctggagat gctgctttgc ctggctgggc 361 cggcctcggg cccgcgctcg ctggctgagc tcctgccacg gccggctgtg gacccccgcg 421 gcctggggca acccttcctg gtgcccgtgc ccgcccggcc gcctctgacc aggggccagt 481 tcgaggaggc ccgggcccac tggcccacgt ccttccacga ggacaagcag gtgaccagcg 541 ccctggctgg gcggctcttc tccacgcagg agcgcgccgc catgcagagc cacatggagc 601 gggcggtgtg ggcggcccgg cgggcagcag cgcggggctt gcgggccgtg ggggccgtgg 661 tagtggaccc ggcctcggac cgcgtgctgg ccaccggcca cgactgcagc tgcgcggaca 721 accccctcct gcacgccgtc atggtgtgcg tggacctcgt ggcgcgcggc cagggccgcg 781 gcacctacga cttcagaccc ttccccgccT GCTCCTTCGC CCCGGCCGCT GCCCCCCAGG 841 CCGTCCGCGC AGGCGCCGTG CGTAAACTGG ACGCAGACGA GGACGGCCTC CCCTACCTGT 901 GCACTGGCTA CGACCTGTAC GTGACCCGCG AGCCCTGCGC CATGTGCGCC ATGGCCCTGG 961 TGCACGCACG CATCCTGCGC GTCTTCTACG GTGCGCCCTC GCCCGACGGC GCCCTGGGCA 1021 CCCGCTTCCG CATCCACGCA CGGCCCGACC TCAACCACCG CTTCCAGGTG TTCCGCGGGG 1081 TGCTGGAGGA GCAGTGCCGC TGGCTGGACC CCGACACGTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAGGGTAC CAGTGCTCAA TCACCTCCAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt