Construct: ORF TRCN0000476255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002815.1_s317c1
- Derived from:
- ccsbBroadEn_07903
- DNA Barcode:
- TGCGTGATCGACGCGGTCCCAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNAPIN (23557)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23557 | SNAPIN | SNAP associated protein | NM_012437.6 | 99.7% | 99.2% | 4G>A |
| 2 | human | 23557 | SNAPIN | SNAP associated protein | NR_052019.1 | 34.2% | (many diffs) | |
| 3 | human | 23557 | SNAPIN | SNAP associated protein | NR_052020.1 | 32.8% | (many diffs) | |
| 4 | mouse | 20615 | Snapin | SNAP-associated protein | NM_133854.3 | 89.7% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gggggctggt tccgccgctg tatcgggggc agggaccccg gtggcggggc 121 ccacaggccg cgaccttttc gccgaagggc tgctggagtt ccTGCGACCC GCTGTGCAGC 181 AGCTCGACTC TCACGTACAC GCCGTCAGAG AGAGCCAGGT AGAGCTCCGG GAACAAATTG 241 ACAACCTAGC CACAGAACTG TGCCGCATAA ATGAGGATCA GAAGGTGGCC CTGGATCTTG 301 ACCCCTATGT TAAGAAGCTA CTTAATGCCC GGCGACGCGT TGTCTTGGTT AACAACATTC 361 TACAGAATGC TCAGGAACGA CTGAGACGGC TAAACCACAG TGTTGCCAAG GAAACAGCCC 421 GCAGGAGAGC AATGCTGGAT TCGGGAATTT ACCCCCCTGG CTCCCCAGGC AAATACCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAT GCGTGATCGA CGCGGTCCCA GGCACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att