Construct: ORF TRCN0000476264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009412.1_s317c1
- Derived from:
- ccsbBroadEn_10774
- DNA Barcode:
- TGCCCGTCTGATACAAGGACAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEFA4 (1669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1669 | DEFA4 | defensin alpha 4 | NM_001925.3 | 99.6% | 100% | 255T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 360
- ORF length:
- 291
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GAGGATTATC GCCCTCCTCG CTGCTATTCT CTTGGTAGCC CTCCAGGTCC 121 GGGCAGGCCC ACTCCAGGCA AGAGGTGATG AGGCTCCAGG CCAGGAGCAG CGTGGGCCAG 181 AAGACCAGGA CATATCTATT TCCTTTGCAT GGGATAAAAG CTCTGCTCTT CAGGTTTCAG 241 GCTCAACAAG GGGCATGGTC TGCTCTTGCA GATTAGTATT CTGCCGGCGA ACAGAACTTC 301 GTGTTGGGAA CTGCCTCATT GGCGGTGTGA GTTTCACATA CTGCTGCACG CGTGTCGATT 361 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 421 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 481 TTTATATATC TTGTGGAAAG GACGATGCCC GTCTGATACA AGGACAAAGA CGCGTTAAGT 541 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt