Construct: ORF TRCN0000476306
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010371.1_s317c1
- Derived from:
- ccsbBroadEn_03594
- DNA Barcode:
- GAACGTAACCCTTACGGTTGGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF19 (55504)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476306
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | NM_001204458.3 | 100% | 100% | |
| 2 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | NM_148957.4 | 100% | 100% | |
| 3 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | NM_018647.5 | 98.5% | 98.1% | 1247delT;1251_1257delGCAGCGA;1260_1269delGGGTTCCCTG |
| 4 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | XM_005266445.3 | 98.5% | 98.1% | 1247delT;1251_1257delGCAGCGA;1260_1269delGGGTTCCCTG |
| 5 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | XM_011535146.1 | 98.5% | 98.1% | 1247delT;1251_1257delGCAGCGA;1260_1269delGGGTTCCCTG |
| 6 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | XM_017020651.1 | 98.5% | 98.1% | 1247delT;1251_1257delGCAGCGA;1260_1269delGGGTTCCCTG |
| 7 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | NM_001354985.2 | 75.6% | 70.2% | (many diffs) |
| 8 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | XM_005266446.2 | 75.6% | 70.2% | (many diffs) |
| 9 | human | 55504 | TNFRSF19 | TNF receptor superfamily me... | NM_001204459.1 | 68.3% | 68.3% | 0_1ins396 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1317
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttaaaagtg ctactagaac aagagaaaac gtttttcact cttttagtat 121 tactaggcta tttgtcatgt aaagtgactt gtgaatcagg agactgtaga cagcaagaat 181 tcagggatcg gtctggaaac tgtgttccct gcaaccagtg tgggccaggc atggagttgt 241 ctaaggaatg tggcttcggc tatggggagg atgcacagtg tgtgacgtgc cggctgcaca 301 ggttcaagga ggactggggc ttccagaaat gcaagccctg tctggactgc gcagtggtga 361 accgctttca gaaggcaaat tgttcagcca ccagtgatgc catctgcggg gactgcttgc 421 caggatttta taggaagacg aaacttgtcg gctttcaaga catggagtgt gtgccttgtg 481 gagaccctcc tcctccttac gaaccgcact gtgccagcaa ggtcaacctc gtgaagatcg 541 cgtccacggc ctccagccca cgggacacgg cgctggctgc cgttatctgc agcgctctgg 601 ccaccgtcct gctggccctg ctcatcctct gtgtcatcta ttgtaagaga cagtttatgg 661 agaagaaacc cagctggtct ctgcggtcac aggacattca gtacaacggc tctgagctgt 721 cgtgttttga cagacctcag ctccacgaat atgcccacag agcctgctgc cagtgccgcc 781 gtgactcagt gcagacctgc gggccggtgc gcttgctccc atccatgtgc tgtgaggagg 841 cctgcagccc caacccggcg actcttggtt gtggggtgca ttctgcagcc agtcttcagg 901 caagaaacgc aggcccagcc ggggagatgg tgccgacttt cttcggatcc ctcacgcagt 961 ccatctgtgg cgagtttTCA GATGCCTGGC CTCTGATGCA GAATCCCATG GGTGGTGACA 1021 ACATCTCTTT TTGTGACTCT TATCCTGAAC TCACTGGAGA AGACATTCAT TCTCTCAATC 1081 CAGAACTTGA AAGCTCAACG TCTTTGGATT CAAATAGCAG TCAAGATTTG GTTGGTGGGG 1141 CTGTTCCAGT CCAGTCTCAT TCTGAAAACT TTACAGCAGC TACTGATTTA TCTAGATATA 1201 ACAACACACT GGTAGAATCA GCATCAACTC AGGATGCACT AACTATGAGA AGCCAGCTAG 1261 ATCAGGAGAG TGGTGCTGTC ATCCACCCAG CCACTCAGAC GTCCCTCCAG GAAGCTTACC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GAGAACGTAA CCCTTACGGT TGGCGAACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt